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Differential sensitivity to gliotoxin of virulent and attenuated poliovirus strains and its possible use as a genetic marker

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Summary

Gliotoxin was shown to exert a less inhibitory effect on standard virulent type 1 and 2 poliovirus strains than on attenuated homotypie strains. This did not apply to type 3 standard strains since the virulent Saukett virus proved to be inhibited to the same degree as the attenuated Leon 12a1b, WM III and USOL D bac strains. An increase in gliotoxin-resistance after several intra-human passages of different live polio vaccine strains occurred only in a vaccinee fed the type 1 LSc2ab strain.

A strong correlation between the gliotoxin marker and therct/40 andMS characters was found in type 1 and 2 poliovirus strains isolated from patients and vaccinees. No correlation was observed between thegli marker and thermoinactivation at 50°C or sensitivity to dextran sulphate, guanidine and HBB.

The covariation between therct/40 andgli characters seems to be asymmetrical sincegli r mutants of the LSc2ab strain maintained theirrct/40 − character, whilerct/40 + mutants of the same parental strain displayed increased gliotoxin-resistance. The value of thegli marker for genetic and epidemiological studies is discussed.

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Author notes

  1. Dina Sergiescu

    Present address: Department of Poliomyelitis, Cantacuzino Institute, Bucharest, Romania

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  1. Department of Poliomyelitis, Cantacuzino Institute, Bucharest, Romania

    A. Aubert-Combiescu

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  1. Dina Sergiescu
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  2. A. Aubert-Combiescu
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Sergiescu, D., Aubert-Combiescu, A. Differential sensitivity to gliotoxin of virulent and attenuated poliovirus strains and its possible use as a genetic marker. Archiv f Virusforschung 27, 268–281 (1969). https://doi.org/10.1007/BF01249649

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  • DOI: https://doi.org/10.1007/BF01249649

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