Summary
Simultaneous addition of RNA preparations from two picornaviruses to facilitator compound resulted in greatly reduced numbers of plaques as compared to the number produced by similar concentrations of interferee RNA (that whose plaque formation was not blocked in interference experiments). A similar phenomenon was demonstrated with RNA from biochemical mutants of picornaviruses and their corresponding wild-type viruses. A method of biochemical blocking was shown to be as effective as that using antiserum. Infectivity of RNA preparations was markedly reduced by ultraviolet irradiation, heat, and snake venom phosphodiesterase; however, these agents did not significantly affect interfering capacity of the RNA preparations.
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This investigation was partially completed at Southern Illinois University, supported by Special Research Grant 2-11-12 from the University, and partially at the Section of Virus Research, Department of Pediatrics, University of Kansas Medical Center, Kansas City, Kansas, supported by National Institute of Allergy and Infectious Diseases Grant No. AI-06263.
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Rouhandeh, H., Yau, T. & Lang, P.A. Homotypic and heterotypic interference among picornavirus ribonucleic acids. Archiv f Virusforschung 27, 236–243 (1969). https://doi.org/10.1007/BF01249646
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DOI: https://doi.org/10.1007/BF01249646