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Nucleo-cytoplasmic studies in the development of influenza virus in mammalian cells

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Summary

A non-ionic detergent method of cell fractionation was used to follow the time course of tritiated uridine incorporation into influenza A-NWS virus-induced RNA in a continuous mammalian cell line. The maximum incorporation of tritiated uridine into acid-insoluble RNA of nuclear fractions occurred at 2 to 2.5 hours post infection. A maximum of labelled acid-insoluble RNA of the cytoplasmic fractions was found at 3 to 4 hours post infection which coincided with active synthesis of infectious virus. The effect of influenza A-NWS infection on the rate of tritiated thymidine incorporation into acid-insoluble DNA was found to be an initial depression 1 to 2 hours post infection followed by recovery to near normal values, then a gradual decline until at 8 hours post infection it was approximately 60% of that shown by the control cell cultures.

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Author notes

  1. W. C. Bell

    Present address: Virus Laboratory, Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, Michigan, USA

Authors and Affiliations

  1. Virus Laboratory, Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, Michigan, USA

    H. F. Maassab

Authors
  1. W. C. Bell
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  2. H. F. Maassab
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Additional information

This paper is part of a dissertation submitted byWilliam C. Bell in partial fulfillment of the requirements for the degree of Doctor of Philosophy from the University of Michigan. Supported in part by Graduate Training Grants from Nat. Inst. of Allergy and Infect. Dis., NIH 5 T1 AI-60.

This investigation was conducted in part under the sponsorship of the Commission on Influenza, Armed Forces Epidemiological Board, and was supported by the U.S. Army Medical Research and Development Command, Department of the Army, under research contract DA-49-193-MD-2066.

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Bell, W.C., Maassab, H.F. Nucleo-cytoplasmic studies in the development of influenza virus in mammalian cells. Archiv f Virusforschung 27, 128–137 (1969). https://doi.org/10.1007/BF01249638

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  • DOI: https://doi.org/10.1007/BF01249638

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