Molecular Breeding

, Volume 1, Issue 4, pp 375–387 | Cite as

Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers

  • Satomi Yoshimura
  • Atsushi Yoshimura
  • Nobuo Iwata
  • Susan R. McCouch
  • M. Lleva Abenes
  • Marietta R. Baraoidan
  • Twng Wah Mew
  • Rebecca J. Nelson
Article

Abstract

Four genes of rice,Oryza sativa L., conditioning resistance to the bacterial blight pathogenXanthomonas oryzae pv.oryzae (X. o. pv.oryzae), were tagged by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. No recombinants were observed betweenxa-5 and RFLP marker lociRZ390, RG556 orRG207 on chromosome 5.Xa-3 andXa-4 were linked to RFLP locusXNpb181 at the top of chromosome 11, at distances of 2.3 cM and 1.7 cM, respectively. The nearest marker toXa-10, also located on chromosome 11, was the RAPD locusO072000 at a distance of 5.3 cM. From this study, the conventional map [19, 28] and two RFLP linkage maps of chromosome 11 [14, 26] were partially integrated. Using the RFLP and RAPD markers linked to the resistance genes, we selected rice lines homozygous for pairs of resistance genes,Xa-4 +xa-5 andXa-4 +Xa-10. Lines carryingXa-4 +xa-5 andXa-4 +Xa-10 were evaluated for reaction to eight strains of the bacterial blight pathogen, representing eight pathotypes and three genetic lineages. As expected, the lines carrying pairs of genes were resistant to more of the isolates than their single-gene parental lines. Lines carryingXa-4 +xa-5 were more resistant to isolates of race 4 than were either of the parental lines (‘quantitative complementation’). No such effects were seen forXa-4 +Xa-10. Thus, combinations of resistance genes provide broader spectra of resistance through both ordinary gene action expected and quantitative complementation.

Key words

disease resistance gene combination Oryza sativa L. RAPD RFLP Xanthomonas oryzae pv.oryzae 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Satomi Yoshimura
    • 1
    • 2
  • Atsushi Yoshimura
    • 1
  • Nobuo Iwata
    • 1
  • Susan R. McCouch
    • 3
    • 4
  • M. Lleva Abenes
    • 3
  • Marietta R. Baraoidan
    • 2
  • Twng Wah Mew
    • 2
  • Rebecca J. Nelson
    • 2
  1. 1.Plant Breeding LaboratoryFaculty of Agriculture, Kyushu UniversityFukuokaJapan
  2. 2.Division of Entomology and Plant PathologyInternational Rice Research InstituteLos BañosPhilippines
  3. 3.Division of Plant Breeding, Genetics and BiochemistryInternational Rice Research InstituteLos BañosPhilippines
  4. 4.Plant Breeding DepartmentCornell UniversityIthacaUSA

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