Skip to main content
Log in

Electrochemical biosensors based on enzymes immobilized in electropolymerized films

  • Original Papers
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A review based on 135 references concerns the design and properties of electrochemical biosensors for 13 different substrates of enzymatic reactions. In the sensors discussed the enzymes are immobilized within or on the top of electropolymerized films, mostly of conducting polymers. Amperometric detection is most often used for internal electrochemical sensing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. H. Shirikawa,Jpn. J Appl. Phys. Supp. (Japan) 1982, 473.

  2. H. Naarmann, N. Theophilou,Synth. Met. 1987,22, 1.

    Google Scholar 

  3. R. W. Lenz, C.-C. Han, J. Stenger-Smith, F. E. Karasz,J. Polym. Sci. (A), Polym. Chem. 1988,26, 3241.

    Google Scholar 

  4. M. Kobayashi, J. Chen, T.-C. Chung, F. Moraes, A. Heeger, F. Wudl,Synth. Met. 1984,9, 77.

    Google Scholar 

  5. N. R. Ratcliffe,Anal. Chim. Acta 1990,239, 257.

    Google Scholar 

  6. A. F. Diaz, K. K. Kanazawa, G. P. Gardini,J. Chem. Soc. Chem. Commun. 1979, 635.

  7. S. A. Wring, J. P. Hart,Analyst 1992,117, 1215.

    Google Scholar 

  8. G. Bidan,Sens. Actuators B 1992,6, 45.

    Google Scholar 

  9. P. R. Teasdale, G. G. Wallace,Analyst 1993,118, 329.

    Google Scholar 

  10. P. N. Bartlett, J. M. Cooper,J. Electroanal. Chem. 1993,362, 1.

    Google Scholar 

  11. M. Trojanowicz, M. L. Hitchman, K. Cammann, in:Current Topics in Biophysics (U. J. Krull, D. P. Nikolelis, eds.), in press.

  12. A. O. Patil, A. J. Heeger, F. Wudl,Chem. Rev. 1988,88, 183.

    Google Scholar 

  13. J. S. Miller,Adv. Mater. 1993,5, 587.

    Google Scholar 

  14. J. S. Miller,Adv. Mater. 1993,5, 671.

    Google Scholar 

  15. J. L. Brédas, B. Thémans, J. J. M. André, R. R. Chance, R. Silbey,Synth. Met. 1984,9, 265.

    Google Scholar 

  16. H. Shinohara, T. Chiba, M. Aizawa,Sens. Acturators 1987,13, 79.

    Google Scholar 

  17. P. C. Pandey,J. Chem. Soc. Faraday Trans. I 1988,84, 2259.

    Google Scholar 

  18. S. Asavapiriyanont, G. K. Chandler, G. A. Gunawardena, D. Pletcher,J. Electroanal. Chem. 1984,177, 229.

    Google Scholar 

  19. M. Freund, L. Bodalbhai, A. Brajter-Toth,Talanta 1991,38, 95.

    Google Scholar 

  20. H. Shinohara, M. Aizawa, H. Shirokawa,J. Chem. Soc. Chem. Commun. 1986, 87.

  21. J. Wang, S.-P. Chen, M. S. Lin,J. Electroanal. Chem. 1989,273, 231.

    Google Scholar 

  22. M. Hämmerle, W. Schuhmann, H.-L. Schmidt,Sens. Actuators B 1992,6, 106.

    Google Scholar 

  23. J. Wang, R. Li,Anal. Chem. 1989,61, 2809.

    PubMed  Google Scholar 

  24. C. Malitesta, F. Palmisano, L. Torsi, P. G. Zambonin,Anal. Chem. 1990,62, 2735.

    PubMed  Google Scholar 

  25. D. Centonze, A. Guerrieri, C. Malitesta, F. Palmisano, P. G. Zambonin,Ann. Chim. (Rome) 1992,82, 219.

    Google Scholar 

  26. J. Wang, H. Wu,Anal. Chim. Acta 1993,283, 683.

    Google Scholar 

  27. D. Centonze, A. Guerrieri, F. Palmisano, L. Torsi, P. G. Zambonin,Fresenius J. Anal. Chem. 1994,349, 497.

    Google Scholar 

  28. M. E. G. Lyons, C. H. Lyons, C. Fitzgerald, T. Bannon,Analyst 1993,118, 361.

    Google Scholar 

  29. A. Lewenstam, J. Bobacka, A. Ivaska,J. Electroanal. Chem. 1994,368, 23.

    Google Scholar 

  30. J. Bobacka, Z. Gao, A. Ivaska, A. Lewenstam,J. Electroanal. Chem. 1994,368, 33.

    Google Scholar 

  31. A. Michalska, A. Lewenstam, A. Ivaska, A. Hulanicki,Electroanalysis 1993,5, 261.

    Google Scholar 

  32. S. Dong, G. Che,Talanta 1991,38, 111.

    Google Scholar 

  33. S. Dong, Z. Sun, Z. Lu,Analyst 1988,113, 1525.

    Google Scholar 

  34. R. Qian, Y. Li, B. Yan, H. Zhang,Synth. Met. 1989, C51.

  35. S. Dong, Z. Sun, Z. Lu,J. Chem. Soc. Chem. Commun. 1988, 993.

  36. A. Cadogan, A. Lewenstam, A. Ivaska,Talanta 1992,39, 617.

    Google Scholar 

  37. A. Hulanicki, A. Michalska, A. Lewenstam,Talanta 1994,41, 323.

    Google Scholar 

  38. Z. Lu, Z. Sun, S. Dong,Electroanalysis 1989,1, 271.

    Google Scholar 

  39. Q. Pei, R. Qian,Electrochim. Acta 1992,37, 1075.

    Google Scholar 

  40. T. Okada, H. Hayashi, K. Hiratani, H. Sugihara, N. Koshizaki,Analyst 1991,116, 923.

    Google Scholar 

  41. O. Inganäs, R. Erandsson, C. Nylander, I. Lundström,J. Phys. Chem. Solids 1984,45, 427.

    Google Scholar 

  42. E. M. Génies, A. A. Syed,Synth. Met. 1984,10, 21.

    Google Scholar 

  43. Q. Pei, R. Qian,Synth. Met. 1991,45, 35.

    Google Scholar 

  44. J. C. Cooper, E. A. H. Hall,Biosens. Bioelectron. 1992,7, 473.

    Google Scholar 

  45. M. Nishizawa, T. Matsue, I. Uchida,Anal. Chem. 1992,64, 2642.

    PubMed  Google Scholar 

  46. M. Trojanowicz, O. Geschke, T. Krawczyński vel Krawczyk, B. Gründig, K. Cammann,Sens. Actuators in press.

  47. M. Trojanowicz, W. Matuszewski, B. Szczepańczyk, A. Lewenstam, in:Uses of Immobilized Biological Compounds (G. G. Guibault, M. Mascini, eds.), Kluwer, Dordrecht, 1993, p. 141.

    Google Scholar 

  48. P. C. Pandey, A. P. Mishra,Analyst 1988,113, 329.

    Google Scholar 

  49. B. F. Y. Yon Hin, R. S. Sethi, C. R. Lowe,Sens. Actuators B 1990,1, 550.

    Google Scholar 

  50. M. Trojanowicz, A. Lewenstam, T. Krawczyński vel Krawczyk, I. Lähdesmäki, W. Szczepek,Electroanalyis in press.

  51. G. Gustafsson, I. Lundström, B. Liedberg, C. R. Wu, O. Inganäs, O. Wennerström,Synth. Met. 1989,31, 163.

    Google Scholar 

  52. P. de Taxis du Poet, S. Miyamoto, T. Murakami, J. Kimura, I. Karube,Anal. Chim. Acta 1990,235, 255.

    Google Scholar 

  53. Y. Degani, A. Heller,J. Am. Chem. Soc. 1989,111, 2357.

    Google Scholar 

  54. A. Heller,Acc. Chem. Res. 1990,23, 128.

    Google Scholar 

  55. B. A. Gregg, A. Heller,Anal. Chem. 1990,62, 258.

    PubMed  Google Scholar 

  56. L. Gorton, H. I. Karan, P. D. Hale, T. Inagaki, Y. Okamoto, T. A. Skotheim,Anal. Chim. Acta 1990,228, 29.

    Google Scholar 

  57. P. D. Hale, T. Inagaki, H. S. Lee, H. I. Karan, Y. Okamoto, T. A. Skotheim,Anal. Chim. Acta 1990,228, 31.

    Google Scholar 

  58. M. Umana, J. Waller,Anal. Chem. 1986,58, 2979.

    Google Scholar 

  59. N. C. Foulds, C. R. Lowe,J. Chem. Soc., Faraday Trans. I 1986,82, 1259.

    Google Scholar 

  60. D. Bélanger, J. Nadreau, G. Fortier,J. Electroanal. Chem. 1989,274, 143.

    Google Scholar 

  61. M. Trojanowicz, W. Matuszewski, M. Podsiadła,Biosens. Bioelectron. 1990,5, 149.

    PubMed  Google Scholar 

  62. D. Bélanger, E. Brassard, G. Fortier,Anal. Chim. Acta 1990,228, 311.

    Google Scholar 

  63. G. Fortier, E. Brassard, D. Bélanger,Biosens. Bioelectron. 1990.5, 473.

    PubMed  Google Scholar 

  64. D. R. Yaniv, L. McCormick, J. Wang, N. Naser,J. Electroanal. Chem. 1991,314, 353.

    Google Scholar 

  65. D. Bélanger, J. Nadreau, G. Fortier,Electroanalysis 1992,4, 933.

    Google Scholar 

  66. D. Centonze, A. Guerrieri, C. Malitesta, F. Palmisano, P. G. Zambonin,Fresenius J. Anal. Chem. 1992,342, 729.

    Google Scholar 

  67. T. Tatsuma, T. Watanabe, T, Watanabe,Sens. Actuators B 1993,14, 752.

    Google Scholar 

  68. C. Iwakura, Y. Kajiya, H. Yoneyama,J. Chem. Soc. Chem. Commun. 1988, 1019.

  69. P. Janda, J. Weber,J. Electroanal. Chem. 1991,300, 119.

    Google Scholar 

  70. M. Hämmerle, W. Schuhmann, H.-L. Schmidt,Sens. Actuators B 1992,6, 106.

    Google Scholar 

  71. M. Marchesiello, M. Génies,Electrochim. Acta 1992,37, 1987.

    Google Scholar 

  72. J. M. Cooper, D. Bloor,Electroanalysis 1993,5, 883.

    Google Scholar 

  73. M. Yasuzawa, N. Matsushita, H. Satake, A. Kunugi,Sens. Actuators B 1993,14, 665.

    Google Scholar 

  74. P. N. Bartlett, R. G. Whitaker,J. Electronal. Chem. 1987,224, 37.

    Google Scholar 

  75. P. N. Bartlett, Z. Ali, V. Eastwick-Field,J. Chem. Soc., Faraday Trans. 1992,88, 2677.

    Google Scholar 

  76. Q. Pei, R. Qian,J. Electroanal. Chem. 1992,322, 153.

    Google Scholar 

  77. Y. Kajiya, H. Sugai, C. Iwakura, H. Yoneyama,Anal. Chem. 1991,63, 49.

    Google Scholar 

  78. Z. Sun, H. Tachikawa,Anal. Chem. 1992,64, 1112.

    PubMed  Google Scholar 

  79. N. C. Foulds, C. R. Lowe,Anal. Chem. 1988,60, 2473.

    PubMed  Google Scholar 

  80. I. Taniguchi, K. Matsushita, M. Okamoto, J.-P. Collin, J.-P. Sauvage,J. Electroanal. Chem. 1990,280, 221.

    Google Scholar 

  81. D. J. Strike, N. F. de Rooij, M. Koudelka-Hep,Sens. Actuators B 1993,13, 61.

    Google Scholar 

  82. P. N. Bartlett, D. J. Caruana,Analyst 1992,117, 1287.

    Google Scholar 

  83. T. Tatsuma, M. Gondaira, T. Watanabe,Anal. Chem. 1992,64, 1183.

    Google Scholar 

  84. P. N. Bartlett, D. J. Caruana,Analyst 1994,119, 175.

    Google Scholar 

  85. D. T. Hoa, T. N. Suresh Kumar, N. S. Punekar, R. S. Srinivasa, R. Lal, A. Q. Contractor,Anal. Chem. 1992,64, 2645.

    Google Scholar 

  86. L. Coche-Guérente, S. Cosnier, C. Innocent, P. Mailley, J.-C. Moutet, R. M. Morélis, B. Leca, P. R. Coulet,Electroanalysis 1993,5, 647.

    Google Scholar 

  87. T. Tatsuma, T. Watanabe, T. Watanabe,J. Electroanal. Chem. 1993,356, 245.

    Google Scholar 

  88. B. F. Y. Yon Hin, C. R. Lowe,Sens. Acuators B 1992,7, 339.

    Google Scholar 

  89. M. G. Garguilo, N. Huynh, A. Proctor, A. C. Michael,Anal. Chem. 1993,65, 523.

    PubMed  Google Scholar 

  90. B. F. Y. Yon-Hin, M. Smolander, T. Crompton, C. R. Lowe,Anal. Chem. 1993,65, 2067.

    Google Scholar 

  91. C. G. J. Koopal, B. de Ruiter, R. J. M. Nolte,J. Chem. Soc. Chem. Commun. 1991, 1691.

  92. G. Fortier, D. Bélanger,Biotechnol. Bioeng. 1991,37, 854.

    Google Scholar 

  93. W. Schuhmann, R. Lammert, B. Uhe, H.-L. Schmidt,Sens. Acturators. B 1990,1, 537.

    Google Scholar 

  94. T. Schalkhammer, E. Mann-Buxbaum, F. Pittner, G. Urban,Sens. Actuators B 1991,4, 273.

    Google Scholar 

  95. E. Wolowacz, B. F. Y. Yon Hin, C. R. Lowe,Anal. Chem. 1992,64, 1541.

    Google Scholar 

  96. S. V. Sasso, R. J. Pierce, R. Walla, A. M. Yacynych,Anal. Chem. 1990,62, 1111.

    Google Scholar 

  97. S. Dong, Q. Deng, G. Cheng,Anal. Chim. Acta 1993,279, 235.

    Google Scholar 

  98. G. F. Khan, E. Kobatake, H. Shinohara, Y. Ikariyama, M. Aizawa,Anal. Chem. 1992,64, 1254.

    Google Scholar 

  99. C. G. J. Koopal, M. C. Feiters, R. J. M. Nolte, B. de Ruiter, R. B.M. Schasfoort,Biosens. Bioelectron. 1992,7, 461.

    Google Scholar 

  100. C. G. J. Koopal, M. C. Feiters, R. J. M. Nolte, B. de Ruiter, R. B. M. Schasfoort,Bioelectrochem. Bioenerg. 1992,29, 159.

    Google Scholar 

  101. R. Czajka, C. G. J. Koopal, M. C. Feiters, J. W. Gerritsen, R. J. M. Nolte, H. Van Kempen,Bioelectrochem. Bioenerg. 1992,29, 47.

    Google Scholar 

  102. C. G. J. Koopal, R. J. M. Nolte,Bioelectrochem. Bioenerg. 1994,33, 45.

    Google Scholar 

  103. S. Kuwabata, C. R. Martin,Anal. Chem. 1994,66, 2757.

    Google Scholar 

  104. A. Begum, H. Tsushima, T. Suzawa, H. Shinohara, Y. Ikariyama, M. Aizawa,Sens. Actuators B 1993,14, 576.

    Google Scholar 

  105. F. Palmisano, D. Centonze, A. Guerrieri, P. G. Zambonin,Biosens. Bioelectron. 1993,8, 393.

    Google Scholar 

  106. E. Tamiya, I. Karube, S. Hattori, M. Suzuki, K. Yokoyama,Sens. Actuators 1989,18, 297.

    Google Scholar 

  107. K. Yokoyama, E. Tamiya, I. Karube,Anal. Lett. 1989,22, 2949.

    Google Scholar 

  108. G. Fortier, D. Bélanger,Biotechnol. Bioeng. 1991,37, 854.

    Google Scholar 

  109. L. D. Couves, S. J. Porter,Synth. Met. 1989,28, C761.

    Google Scholar 

  110. S. J. Porter,Ext. Abstr. ICMS'88, Santa Fe, NM, USA, June 26–July 2, 1988, p. 70.

  111. A. B. Gavrilov, A. F. Zueva, O. N. Efimov, V. A. Bogdanovskaya, M. R. Tarasevitch,Synth. Met. 1993,60, 159.

    Google Scholar 

  112. P. N. Bartlett, R. G. Whitaker,J. Electroanal. Chem. 1987,224, 27.

    Google Scholar 

  113. S. Mu, H. Xue, B. Qian,J. Electroanal. Chem. 1991,304, 7.

    Google Scholar 

  114. J. P. Lowry, R. D. O'Neill,Anal. Chem. 1992,64, 453.

    PubMed  Google Scholar 

  115. F. Palmisano, P. G. Zambonin,Anal. Chem. 1993,65, 2690.

    Google Scholar 

  116. P. N. Bartlett, P. Tebbutt, C. H. Tyrrell,Anal. Chem. 1992,64, 138.

    Google Scholar 

  117. C. Galiatsatos, Y. Ikariyama, J. E. Mark, W. R. Heineman,Biosens. Bioelectron. 1990,5, 47.

    Google Scholar 

  118. P. D. Hale, L. I. Boguslavsky, T. Inagaki, H. I. Karan, H. S. Lee, T. A. Skotheim, Y. Okamoto,Anal. Chem. 1991,63, 677.

    Google Scholar 

  119. B. A. Gregg, A. Heller,J. Phys. Chem. 1991,95, 5976.

    Google Scholar 

  120. E. Rohde, E. Dempsey, M. S. Smyth, J. G. Vos, H. Emons,Anal. Chim. Acta 1993,278, 5.

    Google Scholar 

  121. M. Elmgren, S.-E. Lundquist, M. Sharp,J. Electroanal. Chem. 1993,362, 227.

    Google Scholar 

  122. F. M. McArdle, K. C. Persaud,Analyst 1993,118, 419.

    Google Scholar 

  123. Y. Kajiya, R. Tsuda, H. Yoneyama,J. Electroanal. Chem. 1991,301, 155.

    Google Scholar 

  124. W. Trettnak, I. Lionti, M. Mascini,Electroanalysis 1993,5, 753.

    Google Scholar 

  125. M. V. Deshpande, E. A. H. Hall,Biosens. Bioelectron. 1990,5, 431.

    Google Scholar 

  126. Y. Kajiya, H. Matsumoto, H. Yoneyama,J. Electroanal. Chem. 1991,319, 185.

    Google Scholar 

  127. S. Yabuki, F. Mizutani, M. Asai,Biosens. Bioelectron. 1991,6, 311.

    Google Scholar 

  128. U. Wollenberger, V. Bogdanovskaya, S. Bobrin, F. Scheller, M. Tarasevitch,Anal. Lett. 1990,23, 1795.

    Google Scholar 

  129. T. Matsue, N. Kasai, M. Narumi, M. Nishizawa, H. Yamada, I. Uchida,J. Electroanal. Chem. 1991,300, 111.

    Google Scholar 

  130. S. Cosnier, C. Innocent,J. Electroanal. Chem. 1992,328, 361.

    Google Scholar 

  131. S. B. Adeloju, S. J. Shaw, G. G. Wallace,Anal. Chim. Acta 1993,281, 611, 621.

    Google Scholar 

  132. S. Mu, J. Kan, J. Zhou,J. Electroanal. Chem. 1992,334, 121.

    Google Scholar 

  133. S. D. Bindra, Y. Zhang, G. S. Wilson, R. Sternberg, D. R. Thevenot, D. Moatti, G. Reach,Anal. Chem. 1991,63, 1692.

    PubMed  Google Scholar 

  134. J. Wang, P. Tuzhi,Anal. Chem. 1986,58, 2357.

    Google Scholar 

  135. M. Trojanowicz, M. L. Hitchman,Electroanalysis in press.

Download references

Author information

Authors and Affiliations

Authors

Additional information

Dedicated to our late colleague Wojciech Matuszewski

Rights and permissions

Reprints and permissions

About this article

Cite this article

Trojanowicz, M., Krawczyński vel Krawczyk, T. Electrochemical biosensors based on enzymes immobilized in electropolymerized films. Mikrochim Acta 121, 167–181 (1995). https://doi.org/10.1007/BF01248249

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01248249

Key words

Navigation