Abstract
Plastic disposable choline biosensors based on ruthenized-carbon screen-printed electrodes were prepared and their use for monitoring organophosphorus pesticides and carbamates is described. The presence of 0.5% ruthenium on activated carbon mixed to form a simple graphite-based ink for the working electrode surface increased the sensitivity towards hydrogen peroxide. The choline biosensor is based on such an electrode coupled with choline oxidase immobilized by adsorption and was used to detect the inhibition effect of carbamates and organophosphorus pesticides on acetylcholinesterase. With the optimized procedure described (pH, buffer composition, incubation time, substrate concentration), concentrations of pesticides (Carbofuran) as low as 1 nM could be detected.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
U. Bilitewski, P. Rüger, R. D. Schmid,Biosens. Bioelectron. 1991,6, 369.
M. Prudenziati, in:Handbook of Sensors and Actuators: Thick Film Sensors, Vol. 1 (M. Prudenziati, ed.), Elsevier, The Netherlands, 1994, p. 113.
S. A. Wring, J. P. Hart, B. J. Birch,Analyst 1991,116, 123.
M. F. Cardosi, A. P. F. Turner, in:Advances in Biosensors, Vol. 1 (A. P. F. Turner, ed.) JAI, London, 1991, p. 125.
B.-T. Tay, K.-P. Ang, H. Gunasingham,Analyst 1988,113, 617.
M. F. Cardosi, S. W. Birch,Anal. Chim. Acta 1993,276, 69.
S. F. White, A. P. F. Turner, R. D. Schmid, U. Bilitewski, J. Bradley,Electroanalysis 1994,6, 625.
S. F. White, A. P. F. Turner, U. Bilitewski, R. D. Schmid, J. Bradley,Anal. Chim. Acta 1994,295, 243.
C. Tran-Minh, P. C. Pandey, S. Kumaran,Biosens. Bioelectron. 1990,5, 461.
L. H. Goodson, W. B. Jacobs,Meth. Enzymol. 1976,44, 647.
G. G. Guilbault, J. Ngeh-Ngwainbi, in:Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses (G. G. Guilbault, M. Mascini, eds.) Reidel, Dordrecht, 1988, p. 187.
O. S. Wolfbeis, E. Koller, in:GBF Monographs, Vol. 13 (R. D. Schmid, F. Scheller, eds.) VCH, New York, 1989, p. 221.
W. Höbel, J. Polster,Fresenius J. Anal. Chem. 1992,343, 101.
U. Wollenberger, U. Löffler, R. Gruß, N. Göpel, F. W. Scheller, in:GBF Monographs, Vol. 17 (F. Scheller, R. D. Schmid, eds.) VCH, New York, 1992, p. 35.
J. -L. Marty, K. Sode, I. Karube,Electroanalysis 1992,4, 249.
M. Bernabei, S. Chiavarini, C. Cremisini, G. Palleschi,Biosens. Bioelectron. 1993,8, 265.
J. Kulys, E. J. D'Costa,Biosens. Bioelectron. 1991,6, 109.
P. Skladal, M. Mascini,Biosens. Bioelectron. 1992,7, 335.
G. Palleschi, M. Bernabei, C. Cremisini, M. Mascini,Sens. Actuators B 1992,7, 513.
M. Mascini, F. Pallozzi, A. Liberti,Anal. Chim. Acta 1973,64, 126.
P. N. Bartlett, P. Tebbutt, C. H. Tyrrell,Anal. Chem. 1992,64, 138.
M. Bernabei, C. Cremisini, M. Mascini, G. Palleschi,Anal. Lett. 1991,24, 1317.
(C. R. Worthing, R. J. Hance (eds.),The Pesticide Manual, 9th Ed., British Crop Production Council, 1991.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cagnini, A., Palchetti, I., Mascini, M. et al. Ruthenized screen-printed choline oxidase-based biosensors for measurement of anticholinesterase activity. Mikrochim Acta 121, 155–166 (1995). https://doi.org/10.1007/BF01248248
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01248248