Summary
The effects of different membrane preparations and assay conditions on [3H]5-HT binding to post-mortem human cortical tissue was studied. Optimal binding necessitated thorough removal of endogenous 5-HT and this was achieved either by hypotonic lysis or by preincubation of the membranes at 37‡C. Calcium chloride (4 mM) increased specific [3H]5-HT binding. The further addition of ascorbic acid (5.7 mM) or ascorbic acid and clorgyline (10 ΜM) reduced specific [3H]5-HT binding.
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Cheetham, S.C., Horton, R.W. [3H]5-HT binding in post-mortem human cerebral cortex: methodological considerations. J. Neural Transmission 78, 53–59 (1989). https://doi.org/10.1007/BF01247113
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DOI: https://doi.org/10.1007/BF01247113