Indirect spectrophotometric determination of benzylpenicillin and cloxacillin in pharmaceutical preparations

Abstract

An indirect spectrophotometric method for the determination of benzylpenicillin and cloxacillin is based on extraction of iodine produced by reduction of potassium iodate in acidic medium, where hydrolytic cleavage of a β-lactam is achieved with sodium hydroxide. Beer's law is obeyed within the concentration ranges 0.04–0.8 mg ml−1 benzylpenicillin and 0.02–0.5 mg ml−1 cloxacillin. The precision, accuracy of the method and the effect of foreign substances were studied. The results have been statistically compared with those using the ammonium vanadate method. An oxidation mechanism is proposed.

This is a preview of subscription content, access via your institution.

References

  1. [1]

    R. K. Gilpin,Anal. Chem. 1993,65, 118R.

    Google Scholar 

  2. [2]

    R. K. Gilpin,Anal. Chem. 1989,61, 194R.

    Google Scholar 

  3. [3]

    R. K. Gilpin,Anal. Chem. 1985,57, 31R.

    Google Scholar 

  4. [4]

    R. K. Gilpin,Anal. Chem. 1981,53, 144R.

    Google Scholar 

  5. [5]

    C. A. Janicki, R. K. Gilpin, E. S. Moyer, R. H. Almond, R. H. Erlich,Anal. Chem. 1977,49, 113R.

    Google Scholar 

  6. [6]

    S. Belal, M. A.-H. Elsayed, M. E. Abdel-Hamid, H. Abdine,J. Pharm. Sci. 1981,70, 127.

    Google Scholar 

  7. [7]

    B. S. Sarma, R. Raw, K. B. Raju, D. J. Kanaka,Indian Drugs,1989,26, 309.

    Google Scholar 

  8. [8]

    B. Morelli, M. Maraini, M. Gesmundo,Anal. Lett. 1987,20, 1429.

    Google Scholar 

  9. [9]

    R. C. Miremath, S. M. Mayanna,Mikrochim. Acta 1986,1, 265.

    Google Scholar 

  10. [10]

    C. S. P. Sastry, T. E. Divakar, U. V. Prasad,Chem. Anal. (Warsaw) 1987,32, 301.

    Google Scholar 

  11. [11]

    B. Morelli,Anal. Lett. 1987,20, 141.

    Google Scholar 

  12. [12]

    El-Sebai A. Ibrahim, Y. A. Beltagy, M. M. Abd El-Khalek,Talanta 1977,24, 328.

    Google Scholar 

  13. [13]

    U. Saha,Analyst 1986,111, 1179.

    Google Scholar 

  14. [14]

    I. P. Kaniou, G. A. Zachariadis, J. A. Statis,J. Liq. Chromatogr. 1993,16, 2891.

    Google Scholar 

  15. [15]

    E. W. Kirchmann, L. E. Welch,J. Chromatogr. 1993,633, 111.

    Google Scholar 

  16. [16]

    A. F. Elwalily, F. El-Anwar, M. A. Eid,Alexandria J. Pharm. Sci. 1991,5, 86.

    Google Scholar 

  17. [17]

    S. Z. Yao, J. Shiao, L. H. Nie,Talanta,1989,36, 1249.

    Google Scholar 

  18. [18]

    F. Belal, A. El-Brashy, F. Ibrahim.Microchem. J. 1989,39, 106.

    Google Scholar 

  19. [19]

    M. G. El-Bardicy, M. M. Ellaithy, M. W. Ibrahim, A. K. S. Ahmad,J. Assoc. Off. Anal. Chem. 1989,69, 173.

    Google Scholar 

  20. [20]

    B. Örtenblad,Acta Chem. Scand. 1950,4, 158.

    Google Scholar 

  21. [21]

    K. Ilver, O. I. Johansen, F. Reimers,Acta Pharm. Internat. 1950,1, 225.

    Google Scholar 

  22. [22]

    M. R. Pollock,J. Gen. Microbiol. 1961,26, 239.

    Google Scholar 

  23. [23]

    J. K. Grime, B. Tan,Anal. Chim. Acta 1979,105, 361.

    Google Scholar 

  24. [24]

    A. Besada, N. B. Tadros,Mikrochim. Acta 1987,2, 225.

    Google Scholar 

  25. [25]

    M. Raychowdhury, P. Chakrabarti,Anal. Biochem. 1979,95, 413.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Helaleh, M.I.H. Indirect spectrophotometric determination of benzylpenicillin and cloxacillin in pharmaceutical preparations. Mikrochim Acta 129, 29–32 (1998). https://doi.org/10.1007/BF01246845

Download citation

Key words

  • benzylpenicillin
  • cloxacillin
  • spectrophotometry
  • pharmaceutical analysis