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Electroconvulsive treatment: Effects on phospholipase C activity and GTP binding activity in rat brain

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Summary

The effect of electroconvulsive treatment (ECT) on activities of phospholipase C hydrolyzing phosphatidylinositol (PI-PLC) and phosphatidylinositol 4,5-bisphosphate (PIP2-PLC) and guanosine-5′-(3-O-thio)triphosphate (GTPγ S) binding activity were examined in membrane and cytosol fractions from four discrete areas (prefrontal cortex, hippocampus, striatum, and amygdala) of the rat brain. A single ECT resulted in an increase in cytosolic activities of PI-PLC in the prefrontal cortex and of PIP2PLC in all 4 brain regions examined. There were no significant changes in either PI-PLC or PIP2-PLC activity in membrane fractions after a single ECT. Repeated ECT caused regionally specific changes in PLC activities as follows: in the prefrontal cortex, both cytosolic PI-PLC and PIP2-PLC and membranous PI-PLC activities were decreased; in the hippocampus, no changes in any PLC activities were seen; in the striatum, only membranous PI-PLC activities were increased; and, in the amygdala, cytosolic and membranous PI-PLC and cytosolic PIP2-PLC activities were increased. The pattern of changes in GTPγ S binding activity following repeated ECT resembled those found in PLC activity as follows: in the prefrontal cortex, GTPγ S binding activities were significantly reduced in both membrane and cytosol; in the hippocampus, the activity was decreased in membrane; in the striatum, no changes in GTPγ S binding activity were seen in any fraction; and, in the amygdala, the activity was increased in cytosol. These findings suggest that ECT has complex effects on the G protein-phospholipase C system, possibly affecting neuronal signal transduction.

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Author notes

  1. A. Nishida

    Present address: Department of Neurology and Psychiatry, Gifu University School of Medicine, Tsukasamachi, Gifu, Japan

Authors and Affiliations

  1. Department of Neurology and Psychiatry, Gifu University School of Medicine, Tsukasamachi, Gifu, Japan

    H. Kaiya & S. Wakabayashi

  2. Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi, Gifu, Japan

    T. Tohmatsu & Y. Nozawa

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  1. A. Nishida
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  2. H. Kaiya
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  3. T. Tohmatsu
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  4. S. Wakabayashi
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  5. Y. Nozawa
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Nishida, A., Kaiya, H., Tohmatsu, T. et al. Electroconvulsive treatment: Effects on phospholipase C activity and GTP binding activity in rat brain. J. Neural Transmission 81, 121–130 (1990). https://doi.org/10.1007/BF01245832

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  • DOI: https://doi.org/10.1007/BF01245832

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