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Differentiation of reoviruses and picornaviruses by density gradient centrifugation

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Summary

The two HA moieties of reovirus (infectious particle and top component) were recentrifuged in a CsCl gradient. They were stable under these conditions and purified to such an extent that the top component HA moiety contained only 4 TCD50 per unit. Mixtures of poliovirus and reovirus (all three prototypes) were centrifuged in a CsCl density gradient. The two viruses were clearly separated; the reoviruses accumulated at a density of 1.375 g/ml. and the poliovirus at a density of 1.34 g/ml. A 100-fold relative enrichment of each virus was achieved in these density bands respectively. A similar separation of ECHO-virus 19 and reovirus 2 infectivity and hemagglutinins associated with the infectious particle was obtained, however the noninfectious (top component) hemagglutinin of these two viruses could not be separated, they both accumulated at a density of 1.30 g/ml.

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Author notes

  1. Reto Engler

    Present address: Section of Virus Research, and Department of Biochemistry, Kansas University Medical Center, Kansas City, Kansas, USA

Authors and Affiliations

  1. Section of Virus Research, and Department of Biochemistry, Kansas University Medical Center, Kansas City, Kansas, USA

    M. Taher A. Fouad

Authors
  1. Reto Engler
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  2. M. Taher A. Fouad
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Additional information

This investigation was supported by Public Health Grant No. AI-06263 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health.

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Engler, R., Fouad, M.T.A. Differentiation of reoviruses and picornaviruses by density gradient centrifugation. Archiv f Virusforschung 20, 29–38 (1967). https://doi.org/10.1007/BF01245767

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  • DOI: https://doi.org/10.1007/BF01245767

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