Abstract
The effect of an activated H-ras oncogene on the progression of neoplasia was studied in transformed rat tracheal epithelial cells. Nude mouse tumours produced by injection of these cells exhibited a higher fraction of cells containing the mutantras gene than did the injected cells, while a subclone that lacked theras mutation was much less tumorigenic than parental cells. Serial passage of one cell line containing aras mutation resulted in an increase in the fraction ofras-mutated cells, which suggests that, in this line,ras activation may confer a selective advantagein vitro as well. However, this was not seen in anotherras-containing line, suggesting the importance of alternative pathways in malignant progression of rat tracheal epithelial cells.
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Abbreviations
- RTE:
-
rat tracheal epithelial
- RFLP:
-
restriction-fragment-length polymorphism
- DMBA:
-
7,12-dimethylbenz[a]anthracene
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Supported by NIH grants CA 52925, CA 13343, and ES 00260 and ACS Award IRG-14-33.
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Cosma, G., Hubbard, F., Jamasbi, R.J. et al. Role of H-ras in the malignant progression of rat tracheal epithelial cells. J Cancer Res Clin Oncol 120, 641–644 (1994). https://doi.org/10.1007/BF01245374
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DOI: https://doi.org/10.1007/BF01245374