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New concept in focusing field-flow fractionation and thin layer isopycnic focusing: Coupling of primary electric field with secondary gravitational force

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Abstract

A new concept of isopycnic focusing exploiting the simultaneous action of coupled electric and gravitational fields is described. Experimental implementation of this concept is demonstrated under conditions of focusing field-flow fractionation and of newly proposed thin layer isopycnic focusing. Both methods are aimed for particle separations. Although the demonstration was performed by using model cross-linked polymer and latex particles, the most important field of potential applications is intended for analytical and preparative separations of biological particles.

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References

  1. J. Janča,Makromol. Chem., Rapid Commun. 1982,3, 887.

    Google Scholar 

  2. J. C. Giddings,Sep. Sci. Technol. 1983,18, 765.

    Google Scholar 

  3. J. Janča,Int. Symp. Polym. Anal. Charact. 1991, May 28–31, Baltimore, USA; J. Appl. Polym. Sci., Appl. Polym. Symp. 1992,51, 91.

  4. J. Janča,Am. Lab. 1992,24, 15.

    Google Scholar 

  5. J. Janča, R. Audebert,Int. Symp. Polym. Anal. Charact. 1992, June 1–4, Inuyama, Japan;J. Appl. Polym. Sci., Appl. Polym. Symp. (in press).

  6. R. J. Hunter,Zeta Potential in Colloid Science, Principles and Applications, Academic Press, London, 1981.

    Google Scholar 

  7. Percoll: Methodology and Applications;Density Marker Beads for Calibration of Gradients of Percoll, Pharmacia Fine Chemicals booklet.

  8. J. Janča,Field-Flow Fractionation: Analysis of Macromolecules and Particles, Chromatogr. Sci. Ser., Vol. 39, Dekker, New York, 1988.

    Google Scholar 

  9. J. Janča, R. Audebert,J. Liquid Chromatogr. (in press).

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Janča, J., Audebert, R. New concept in focusing field-flow fractionation and thin layer isopycnic focusing: Coupling of primary electric field with secondary gravitational force. Mikrochim Acta 111, 163–175 (1993). https://doi.org/10.1007/BF01245303

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  • DOI: https://doi.org/10.1007/BF01245303

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