Abstract
A new concept of isopycnic focusing exploiting the simultaneous action of coupled electric and gravitational fields is described. Experimental implementation of this concept is demonstrated under conditions of focusing field-flow fractionation and of newly proposed thin layer isopycnic focusing. Both methods are aimed for particle separations. Although the demonstration was performed by using model cross-linked polymer and latex particles, the most important field of potential applications is intended for analytical and preparative separations of biological particles.
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J. Janča,Makromol. Chem., Rapid Commun. 1982,3, 887.
J. C. Giddings,Sep. Sci. Technol. 1983,18, 765.
J. Janča,Int. Symp. Polym. Anal. Charact. 1991, May 28–31, Baltimore, USA; J. Appl. Polym. Sci., Appl. Polym. Symp. 1992,51, 91.
J. Janča,Am. Lab. 1992,24, 15.
J. Janča, R. Audebert,Int. Symp. Polym. Anal. Charact. 1992, June 1–4, Inuyama, Japan;J. Appl. Polym. Sci., Appl. Polym. Symp. (in press).
R. J. Hunter,Zeta Potential in Colloid Science, Principles and Applications, Academic Press, London, 1981.
Percoll: Methodology and Applications;Density Marker Beads for Calibration of Gradients of Percoll, Pharmacia Fine Chemicals booklet.
J. Janča,Field-Flow Fractionation: Analysis of Macromolecules and Particles, Chromatogr. Sci. Ser., Vol. 39, Dekker, New York, 1988.
J. Janča, R. Audebert,J. Liquid Chromatogr. (in press).
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Janča, J., Audebert, R. New concept in focusing field-flow fractionation and thin layer isopycnic focusing: Coupling of primary electric field with secondary gravitational force. Mikrochim Acta 111, 163–175 (1993). https://doi.org/10.1007/BF01245303
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DOI: https://doi.org/10.1007/BF01245303