Studies on immunity in poliomyelitis. I. homologous immunity produced with ultraviolet-irradiated adsorbate vaccine

Summary

The A. K. strain of poliomyelitis virus, and the F strain of the Columbia SK group of viruses were adsorbed on aluminium hydroxide or on alu-Archiv f. Virusforschung, Bd. V, H. 1. minium phosphate, and subsequently partly or completely inactivated by ultraviolet irradiation. The adsorbates were inoculated intracerebrally or intramuscularly into rhesus and cynomolgus monkeys. The rate of adsorption on aluminium phosphate was higher (practically 100 percent) than on the hydroxide, and the attachment to the former was extremely fast, so that it did not produce paralysis following intracerebral inoculation. Fifty per cent of the monkeys that received two or three intramuscular injections with 1 ml of the inactivated adsorbate vaccine proved resistant to 100 PD₅₀ of intracerebrally inoculated homologous virus. When vaccinating with attenuated adsorbed virus, 55 to 100 percent of the animals proved resistant to the challenge dose. In vaccinated mice neutralizing antibodies developed. The suitability of similarly prepared vaccines in human beings is discussed.