Summary
Immunocytochemical double labelling was used to determine the structural relationship of oxytocin (OT) and tyrosine hydroxylase (TH) containing perikarya and processes in the rat hypothalamus. Extrahypothalamic TH fibers, as well as parvocellular TH neurons were found to form contacts with OT cells. A fraction of the OT neurons contained TH immunoreactivity. It is likely that in addition to the classical mesencephalic afferences also hypothalamic interneurons and magnocellular dopaminergic neurons control the hypothalamo neurohypophysial system.
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References
Arai R, Guitteny AF, Calas A, Bloch B (1992) Vasopressin mRNA and tyrosine hydroxylase in the paraventricular and supraoptic nuclei of the Brattleboro rat: in situ hybridization histochemical and immunohistochemical studies. In: Wegmann R, Wegmann MA (eds) Recent advances in cellular and molecular biology, 3. Peckten Press, Leuven, pp 3–7
Björklund A, Lindvall O (1984) Dopamine containing systems in the CNS. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy, vol 2. Classical transmitters in the CNS, part 1. Elsevier, Amsterdam, pp 55–122
Buijs RM, Geffard M, Pool CW, Hoorneman EMD (1984) The dopaminergic innervation of the supraoptic and paraventricular nucleus. A light and electron microscopical study. Brain Res 323: 65–72
Bridges TE, Hillhouse EW, Jones MT (1976) The effect of neurohypophyseal hormon release in vivo and from the rat neural lobe and hypothalamus in vivo. J Physiol 260: 647–666
Chan-Palay V, Zaborsky L, Kohler C (1984) Distribution of tyrosine hydroxylase immunoreactive neurons in the hypothalamus of the rat. J Comp Neurol: 467–496
Clarke G, Lincoln DW, Merrick LP (1979) Dopaminergic control of oxytocin release in lactating rats. J Neuroendocrinol 83: 409–420
Decavel C, Geffard M, Calas A (1987) Comparative study of dopamine- and noradrenaline-immunoreactive terminals in the paraventricular and supraoptic nuclei of the rat. Neurosci Lett 77: 149–154
Goldsmith PC, Thind KK, Song T, Kim EJ, Boggan JE (1990) Location of the neuroen-docrine dopamine neurons in the monkey hypothalamus by retrograde tracing and immunostaining. J Neuroendocrinol 2: 169–179
Hökfelt T, Martesson R, Björklund A, Kleinau S (1984) Distributional maps of tyrosine hydroxylase-imrmmoreactive neurons in the rat brain. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy, vol 2. Classical transmitters in the CNS, part 1. Elsevier, Amsterdam, pp 277–379
Holzbauer M, Sharman DF, Godden U (1978) Observations on the function of the dopaminergic nerves innervating the pituitary gland. Neuroscience 3: 1251–1262
Jirikowski GF, Caldwell JD, Pedersen CA, Stumpf WE (1988) Oxytocinergic neuronal systems in the rat hypothalamus are influenced by estrogens. Neuroscience 25: 237–248
Jirikowski GF, Ramalho-Ortigao JF, Caldwell JD (1991) Transitory coexistence of oxytocin and vasopressin in the hypothalamo neurohypophysial system of parturient rats. Horm Metab Res 23: 476–480
Kiss JZ, Mezey E (1986) Tyrosine hydroxylase in magnocellular neurosecretory neurons. Response to physiological manipulations. Neuroendocrinology 43: 519–525
Lindvall O, Björklund A, Skagerberg G (1984) Selective histochemical demonstration of dopamine terminal system in rat di- and telencephalon: new evidence for dopaminergic innervation of hypothalamic neurosecretory nuclei. Brain Res 306: 19–30
Mason WT (1983) Excitation by dopamine on putative oxytocinergic neurons in the rat supraoptic nucleus in vitro: evidence for two classes of continous firing neurons. Brain Res 267: 113–121
Meister B, Villar MJ, Ceccatelli S, Hökfelt T (1990a) Localisation of chemical messengers in magnocellular neurons of the hypothalamic supraoptic and paraventricular nuclei: an immunohistochemical study using experimental manipulations. Neuroscience 37: 603–633
Meister B, Cortes R, Villar MJ, Schalling M, Hökfelt T (1990b) Peptides and transmitter enzymes in hypothalamic magnocellular neurons after administration of hyperosmotic stimuli: comparison between messenger RNA and peptide/protein levels. Cell Tissue Res 260: 279–297
Moos F, Richard P (1979) Effects of dopaminergic antagonist/agonist on oxytocin release induced by various stimuli. Neuroendocrinology 28: 138–144
Moos F, Richard P (1982) Excitatory effect of dopamine on oxytocin and vasopressin reflex releases in the rat. Brain Res 241: 249–260
Neumann I, Russel JA, Landgraf R (1993) Oxytocin and vasopressin release within the supraoptic and paraventricular nuclei of pregnant, parturient, and lactating rats: a microdialysis study. Neuroscience (in press)
Parker SL, Crowley (1992) Activation of central D-1 dopamine receptors stimulates oxytocin release in the lactating rat: evidence for involvement of the hypothalamic paraventricular and supraoptic nuclei. Neuroendocrinology 56: 385–392
Reeves SG, Roberts MA, Siebert STA, Durst RA (1993) Investigation of a novel microtiter plate support material and scanner quantitation of proteins, phospholipids, and immunoassays. Anal Lett (in press)
Sawchenko PE, Swanson LW (1981) Central noradrenergic pathways for the integration of hypothalamic neuroendocrine and autonomic responses. Science 214: 685–687
Swanson LW, Sawchenko PE, Berod A, Hartman BK, Helle KB, Vanorden DE (1981) An immunohistochemical study of the organization of catecholaminergic cells and terminal fields in the paraventricular and supraoptic nuclei of the hypothalamus. J Comp Neurol 196: 271–285
Van den Pol AN, Herbst RS, Powell JF (1984) Tyrosine hydroxylase-immunoreactive neurons of the hypothalamus: a light and electron microscopic study. Neuroscience 13: 1117–1156
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Skutella, T., Weber, T. & Jirikowski, G.F. Coexistence of oxytocin and tyrosine hydroxylase in the rat hypothalamus, an immunocytochemical study. J. Neural Transmission 94, 55–61 (1993). https://doi.org/10.1007/BF01244983
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DOI: https://doi.org/10.1007/BF01244983