Summary
Distribution of Na+K+-ATPase (EC. 3.6.1.3) and its susceptibility to noradrenaline (NA) were studied on electronmicroscopically characterized subcellular fractions of rat brain cortex. The highest specific activity of Na+K+-ATPase was present in synaptosomal fraction disrupted in distilled water (29.52±5.53μmoles phosphate/mg protein/hour). In the presence of 1 mM EGTA significantly higher specific activity was determined in all fractions studied, except homogenate and synaptosome. The effect of NA was studied in concentration range from 10−6-10−3M. 10−4M of NA produced the highest activation of the enzyme in different fractions. Also in the presence of EGTA NA was able to increase the enzyme activity. The effect of NA was much more marked on disrupted synaptosomal fraction. No qualitative differences have been found between the Na+K+-ATPase activities exhibited by the synaptosomal fraction and by other subcellular fractions with respect to susceptibility to NA. Therefore, it seems very probable that NA might modulate neurochemical transmission not only via an effect on nerve terminals but also via stimulating other part of the neuron.
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References
Cantley, L. C. jr., Resh, M. D., Guidotti, G. Vanadate inhibits the red cell (Na+K+) ATPase from the cytoplasmic side. Nature272, 552–554 (1978).
Desaiah, D., Ho, K. K. Effect of biogenic amines and GABA on ATPase activities in mouse tissue. Eur. J. Pharmac.40, 225–261 (1976).
Fiske, C. H., Subbarow, Y. The colorimetric determination of phosphores. J. biol. chem.66, 375–400 (1925).
Garcia, A. G., Kispekar, S. M. Inhibition of Na, K-activated ATPase and release of transmitters. Nature257, 722 (1975).
Gilbert, I. C., Wyllie, M. G., Davison, D. V. Nerve terminal ATPase as possible trigger for neurotransmitter release. Nature255, 237–238 (1975).
Gilbert, I. C., Wyllie, M. G. Effects of anticonvulsant and convulsant drugs on the ATPase activities of synaptosomes and their components. Br. J. Pharmac.56, 49–57 (1976).
Godfraind, G., Koch, M. C., Verbeke, N. The action of EGTA on the catecholamine stimulation of rat brain Na-K-ATPase. Biochem. Pharm.23, 3505–3511 (1974).
Hajós, F. An improved method for the preparation of synaptosomal fractions in high purity. Brain Res.93, 485–499 (1975).
Hexum, T. D. The effect of catecholamines on transport (Na, K) adenosine triphosphatase. Biochem. Pharmac.26, 1221–1227 (1977).
Langer, S. Z. Presynaptic regulation of catecholamine release. Biochem. Pharmacol.23, 1793–1800 (1974).
Logan, J. G., O'Donovan, D. J. The effects of ouabain and the activation of neuronal membrane ATPase by biogenic amines. J. Neurochem.27, 185–189 (1976).
Lee, S. L., Phillis, J. W. Stimulation of cerebral cortical synaptosomal Na-K-ATPase by biogenic amines. Can. J. Pharmac. Physiol.55, 961–964 (1978).
Paton, W. D. M., Vizi, E. S. The inhibitory action of noradrenaline and adrenaline on acetylcholine output by guinea-pig longitudinal muscle strip. Br. J. Pharmac.35, 10–28 (1969).
Schaefer, A., Unyi, G., Pfeifer, A. K. The effects of a soluble factor and of catecholamines on the activity of adenosine triphosphatase in subcellular fractions of rat brain. Biochem. Pharmac.21, 2289–2294 (1972).
Skou, J. C. The influence of some cations on an adenosine triphosphatase from peripheral nerve. Biochem. Biophys. Acta23, 394–400 (1957).
Skou, J. C. Further investigations on a Mg+++Na+ activated adenosine triphosphatase possibly related to the active lin transport of Na+ and K+ across the nerve membrane. Biochem. Biophys. Acta42, 6–23 (1960).
Skou, J. C. Enzymatic basis for active transport of Na+ and K+ across cell membrane. Physiol. Rev.45, 595–617 (1965).
Somogyi, J. Über die Wirkung der Ca-Ionen auf die durch Na+ und K+ aktivierbare Adenosintriphosphatase des Hirngewebes. Ztschr. f. Physiologische Chemie336, 264–270 (1964).
Starke, K. Regulation of noradrenaline release by presynaptic receptor systems. Rev. Physiol. Biochem. Pharmacol.77, 3–124 (1977).
Vizi, E. S. The inhibitory action of noradrenaline and adrenaline from guinea-pig ileum longitudinal strips. Arch. exp. Path. Pharmak.259, 199–200 (1968).
Vizi, E. S. Stimulation by inhibition of (Na+-K+-Mg2) activated ATPase, of acetylcholine release in cortical slices from rat brain. J. Physiol. (London)266, 89–117 (1972).
Vizi, E. S. Termination of transmitter release by stimulation of Na+K+-activated ATPase: role of the sodium pump in triggering action. J. Physiol. (London)267, 261–280 (1977).
Vizi, E. S. Na+K+-activated adenosinetriphosphatase as a trigger in transmitter release. Neuroscience3, 367–384 (1978).
Yoshimura, K. Activation of Na+-K+-activated ATPase in rat brain by catecholamines. J. Biochem.74, 389–391 (1973).
Wu, P. H., Phillis, J. W. Effects ofα- andβ-adrenergic blocking agent on the biogenic amine stimulated (Na+-K+) ATPase of rat cerebral cortical synaptosomal membrane. Gen. Pharmac.9, 421–424 (1978).
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Ádám-Vizi, V., Vizi, E.S. & Horváth, I. Stimulation by noradrenaline of Na+K+ ATPase in different fractions of rat brain cortex. J. Neural Transmission 46, 59–69 (1979). https://doi.org/10.1007/BF01243429
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DOI: https://doi.org/10.1007/BF01243429