Summary
The synthesis of 5-hydroxytryptamine (5-HT) from3H-tryptophan in rat forebrain synaptosomes was studied. Synaptosomes which had been preincubated with3H-tryptophan were perfused with Krebs solution and the release of tryptophan, 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) was determined. K+ depolarization induced a Ca2+-dependent release of 5-HT and tryptophan but had no effect on 5-HIAA efflux. This finding suggests that the release of tryptophan is unlikely to be a non-specific effect of depolarization.
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Collard, K.J., Evans, T.N.W., Suter, H.A. et al. The stimulus-induced release of 5-hydroxytryptamine and tryptophan from superfused rat brain synaptosomes. J. Neural Transmission 53, 223–230 (1982). https://doi.org/10.1007/BF01243414
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DOI: https://doi.org/10.1007/BF01243414