Summary
The injection of reserpine [2.5 mg/kg, intraperitoneally (i.p.)] increased brain concentrations of tyrosine and of the major catecholamine metabolites, 3-methoxy-4-hydroxy-phenylacetic acid (DOPAC), homovamllic acid (HVA), and 3-methoxy-4-hydroxy-phenylethylene-glycol-sulfate (MHPG-SO4) in the rat. In reserpine-treated animals, tyrosine administration (200 mg/kg, i.p.) caused further increases in brain tyrosine, DOPAC, and HVA, but not in brain MHPG-SO4. The increases in DOPAC and HVA levels were observed in a dopaminergic brain region (the corpus striatum) and a noradrenergic region (the cerebellum). These results support previous observations that catecholamine synthesis and release in both dopaminergic and noradrenergic neurons can depend in part upon brain tyrosine levels.
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References
Andén, N.-E., Roos, B.-E., Werdinius, B.: On the occurrence of homovanillic acid in brain and cerebrospinal fluid and its determination by a fluorimetric method. Life Sci.2, 448–458 (1963).
Andén, N.-E., Roos, B.-E., Werdinius, B.: Effects of chlorpromazine, haloperidol and reserpine on the levels of phenolic acids in rabbit corpus striatum. Life Sci.3, 149–158 (1964).
Bareggi, S. R., Genovese, E., Markey, K.: Short- and long-term effects of reserpine on the concentration of 1-(4-hydroxy-3-methoxyphenyl)-ethane-1,2-diol (MOPEG-SO4) in the brain of the rat. Br. J. Pharmacol.65, 573–578 (1979).
Bertler, A., Rosengren, E.: Occurrence and distribution of catechol amines in brain. Acta Physiol. Scand.47, 350–361 (1959).
Bertler, A.: Effect of reserpine on the storage of catechol amines in brain and other tissues. Acta Physiol. Scand.51, 75–83 (1961).
Bunney, B. S., Walters, J. R., Roth, R. H., Aghajanian, G. K.: Dopaminergic neurons: effect of antipsychotic drugs and amphetamine on single cell activity. J. Pharmacol. Exp. Ther.185, 560–571 (1973).
Carlsson, A., Lindqvist, M., Magnusson, T., Waldeck, B.: On the presence of 3-hydroxytyramine in brain. Science127, 471 (1958).
Carlsson, A.: The occurrence, distribution and physiological role of catecholamines in the nervous system. Pharmacol. Rev.11, 490–493 (1959).
Carlsson., A., Lindqvist, M.: Dependence of 5-HT and catecholamine synthesis on concentrations of precursor amino-acids in rat brain. Naunyn-Schmiedeberg's Arch. Pharmacol.303, 157–164 (1978).
Costa, E., Guidotti, A., Zivkovic, B.: Short- and long-term regulation of tyrosine hydroxylase. Adv. Biochem. Psychopharmacol.12, 161–175 (1974).
Felice, L. J., Felice, J. D., Kissinger, P. T.: Determination of catecholamines in rat brain parts by reverse-phase ion-pair liquid chromatography. J. Neurochem.31, 1461–1465 (1978).
Gibson, C. J., Wurtman, R. J.: Physiological control of brain catecholamine synthesis by brain tyrosine concentration. Life. Sci.22, 1399–1406 (1978).
Glowinski, J., Iversen, L. L.: Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H] dopa in various regions of the brain. J. Neurochem.13, 655–669 (1966).
Goldstein, M., Anagnoste, B., Shirron, C.: The effect of trivastal, haloperidol and dibutyryl cyclic AMP on [14C] dopamine synthesis in rat striatum. J. Pharm. Pharmacol.25, 348–351 (1973).
Harris, J. E., Morgenroth, V. H., III, Roth, R. H., Baldessarini, R. J.: Regulation of catecholamine synthesis in the rat brain in vitro by cyclic AMP. Nature252, 156–158 (1974).
Hefti, F.: A simple, sensitive method for measuring 3,4-dihydroxyphenylacetic acid and homovanillic acid in rat brain tissue using high-performance liquid chromatography with electrochemical detection. Life Sci.25, 775–781 (1979).
Hefti, F., Melamed, E., Wurtman, R. J.: Partial lesions of the dopaminergic nigrostriatal system in rat brain: biochemical characterization. Brain Res.195, 123–137 (1980).
Hillarp, N.-A., Fuxe, K., Dahlström, A.: Demonstration and mapping of central neurons containing dopamine, noradrenaline, and 5-hydroxy tryptamine and their reactions to psychopharmaca. Pharmacol. Rev.18, 727–741 (1966).
Holzbauer, M., Vogt, M.: Depression by reserpine of the noradrenaline concentration in the hypothalamus of the cat. J. Neurochem.1, 8–11 (1956).
Iggo, A., Vogt, M.: Preganglionic sympathetic activity in normal and in reserpine-treated cats. J. Physiol. (Lond.)150, 114–133 (1960).
Joh, T. H., Park, D. H., Reis, D. J.: Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation. Proc. Natl. Acad. Sci. USA75, 4744–4748 (1978).
Lovenberg, W., Ames, M. M., Lerner, P.: Mechanisms of acute regulation of tyrosine hydroxylase. In: Psychopharmacology: A Generation of Progress (Lpton, M. A., DiMascio, A., Killam, K. F., eds.), pp. 247–259. New York: Raven Press. 1978.
Meek, J. L., Neff, N. H.: Fluorimetric estimation of 4-hydroxy-3-methoxyphenylethylen-glycol sulphate in brain. Br. J. Pharmacol.45, 435–441 (1972).
Melamed, E., Hefti, F., Wurtman, R. J.: Tyrosine administration increases striatal dopamine release in rats with partial nigrostriatal lesions. Proc. Natl. Acad. Sci. USA77, 4305–4309 (1980).
Nagatsu, T., Levitt, M., Udenfriend, S.: Tyrosine hydroxylase: the initial step in norepinephrine biosynthesis. J. Biol. Chem.239, 2910–2917 (1964).
Potter, J., Axelrod, J.: Subcellular localization of catecholamines in tissues of the rat. J. Pharmacol.142, 291–298 (1963).
Roth, R. H., Morgenroth, R. V. III, Salzman, P. M.: Tyrosine hydroxylase: allosteric activation induced by stimulation of central noradrenergicneurones. Naunyn-Schmiedeberg's Arch. Pharmacol.289, 327–343 (1975).
Scally, M. C., Ulus, I., Wurtman, R. J.: Brain tyrosine levels control striatal dopamine synthesis in haloperidol-treated rats. J. Neural Transm.41, 1–6 (1977).
Sved, A. F., Fernström, J. D., Wurtman, R. J.: Tyrosine administration reduces blood pressure and enhances brain norepinephrine release in spontaneously hypertensive rats. Proc. Natl. Acad. Sci. USA76, 3511–3514 (1979 a).
Sved, A. F., Fernstrom, J. D., Wurtman, R. J.: Tyrosine administration decreases serum prolactin levels in chronically reserpinized rats. Life Sci.25, 1293–1300 (1979 b).
Tagliamonte, A., Tagliamonte, P., Perez-Cruet, J., Stern, S., Gessa, G. L.: Effects of psychotropic drugs on tryptophan concentration in the rat brain. J. Pharmacol. Exp. Ther.177, 475–480 (1971).
Thoenen, H.: Comparison between the effect of neuronal activity and nerve growth factor on the enzymes involved in the synthesis of norepinephrine. Pharmacol. Rev.24, 255–267 (1972).
Vogt, M.: The concentration of sympathin in different parts of the central nervous system under normal conditions and after the administration of drugs. J. Physiol. (Lond.)123, 451–481 (1954).
Waalkes, T. P., Udenfriend, S.: A fluorimetric method for estimation of tyrosine in plasma and tissues. J. Lab. Clin. Med.50, 733–736 (1957).
Wurtman, R. J., Larin, F., Mostafapour, S., Fernstrom, J. D.: Brain catechol synthesis: control by brain tyrosine concentration. Science185, 183–184 (1974).
Wurtman, R. J., Fernstrom, J. D.: Control of brain neurotransmitter synthesis by precursor availability and nutritional state. Biochem. Pharmacol.25, 1691–1696 (1976).
Wurtman, R. J., Scatty, M. C., Gibson, C. J., Hefti, F.: Relation between brain tyrosine and catecholamine synthesis. In: Catecholamines: Basic and Clinical Frontiers (Usdin, E., Kopin, I. J., Barchas, J. D., eds.), pp.64–66. New York: Pergamon Press. 1979.
Zivkovic, B., Guidotti, A.: Changes of kinetic constant of striatal tyrosine hydroxylase elicited by neuroleptics that impair the function of dopamine receptors. Brain Res.79, 505–509 (1974).
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Oishi, T., Wurtman, R.J. Effect of tyrosine on brain catecholamine turnover in reserpine-treated rats. J. Neural Transmission 53, 101–108 (1982). https://doi.org/10.1007/BF01243401
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DOI: https://doi.org/10.1007/BF01243401