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über die antivirale Wirkung einiger Imidazolderivate

Antiviral activity of imidazole derivates. III. Autoradiographic studies on the influence of 1-butyl-4(4′-allyloxyphenyl)-imidazole-hydrochloride on RNA synthesis in mengovirus infected FL cells III. Mitteilung: Autoradiographische Untersuchungen zur Einwirkung von 1-Butyl-4(4′-allyloxyphenyl)-imidazol hydrochlorid auf die RNS-Synthese in FL-Zellen nach Mengovirus-Infektion III. Autoradiographic studies on the influence of 1-butyl-4(4′-allyloxyphenyl)-imidazole-hydrochloride on RNA synthesis in mengovirus infected FL cells

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Zusammenfassung

Autoradiographische Untersuchungen mit3H-Uridin zeigen, da\ das Imidazolderivat 1-Butyl-4(4′-allyloxyphenyl)-imidazol-hydrochlorid (BC 59) in einer Konzentration von 10 mcg/ml die zellulÄre RNS-Synthese in FL-Zellen teilweise unterdrückt. Das Isotop wird nach einem Puls von 60 Minuten bei den Kontrollen fast ausschlie\lich in den Kern eingebaut. Die virale RNS-Synthese nach Mengovirusinfektion findet dagegen im Cytoplasma statt und wird durch den Inhibitor BC59 gehemmt. Bei Applikation unmittelbarp.i. tritt keine cytoplasmatische virus-spezifische Markierung mehr auf.

Summary

Using FL cells after mengovirus infection, autoradiographic investigations with3H-Uridine indicate that the imidazole derivative 1-butyl-4(4′-allyloxyphenyl)-imidazole-hydrochloride (BC59) applied in concentrations of 10 Μg/ml partially inhibits cellular RNA synthesis. After a pulse of one hour the isotop is incorporated generally in the nucleus. On the other hand, the RNA synthesis of mengovirus takes place only in the cytoplasm of the host cell and is blocked completely by the inhibitor BC59. Therefore, after application of this compound no further virus-specific label appears in the cytoplasm.

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Author notes

  1. E. M. Tonew

    Present address: Zentralinstitut für Mikrobiologie und Experimentelle Therapie der Deutschen Akademie der Wissenschaften zu Berlin, Jena, DDR

Authors and Affiliations

  1. Zentralinstitut für Mikrobiologie und Experimentelle Therapie der Deutschen Akademie der Wissenschaften zu Berlin, Jena, DDR

    M. Tonew & G. Strohbach

  2. Bereich Experimentelle Therapie und Bereich Molekularbiologie, Deutschland

    E. M. Tonew, M. Tonew & G. Strohbach

Authors
  1. E. M. Tonew
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  2. M. Tonew
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  3. G. Strohbach
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Tonew, E.M., Tonew, M. & Strohbach, G. über die antivirale Wirkung einiger Imidazolderivate. Archiv f Virusforschung 34, 244–249 (1971). https://doi.org/10.1007/BF01242998

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  • DOI: https://doi.org/10.1007/BF01242998

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