Summary
The morphogenesis of Aujeszky's Disease Virus in synchronously infected pig kidney cells was studied at hourly intervals for 24 hours. Naked capsids were present in the nucleus from 3 hours and in the cytoplasm from 4 hours after infection. Capsids acquired their envelope not only at the inner nuclear membrane but also in the cytoplasm at smooth-membraned vesicles, the outer nuclear membrane, rough endoplasmic reticulum or the Golgi complex. Extracellular mature virus first appeared at 5 hours and after this time most enveloped particles were extracellular. Virus was probably released by fusion of vesicles with the plasma membrane. These results extend previous work on Aujeszky's Disease Virus and in particular, establish the time required for virus morphogenesis.
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References
Achong, B. G., andE. V. Meurisse: Observations on the fine structure and replication of varicella virus in cultivated human amnion cells. J. gen. Virol.3, 305–308 (1968).
Becker, P., J. L. Melnick, andH. D. Mayor: A morphologic comparison between the developmental stages of herpes zoster and human cytomegalovirus. Exp. molec. Path.4, 11–23 (1965).
Darlington, R. W., andL. H. Moss: Herpesvirus envelopment. J. Virol.2, 48–55 (1968).
Darlington, R. W., andL. H. Moss: The envelope of herpesvirus. Progr. med. Virol.11, 16–45 (1969).
De Robertis, E. D. P., andA. P. De Iraldi: Orientation: Submicroscopic Structure. In: Electron Microscopic Anatomy, 1st Edit., pp. 1–36 (S. M. Kurtz, ed.), New York: Academic Press Inc., 1964.
Felluga, B.: Electron microscopic observations on pseudorabies virus development in a line of pig kidney cells. Ann. Sclavo5, 412–424 (1963).
Kaplan, A. S., andA. E. Vatter: A comparison of herpes simplex and pseudorabies viruses. Virology7, 394–407 (1959).
McGavran, M. H., andM. G. Smith: Ultrastructural, cytochemical, and microchemical observations on cytomegalovirus (salivary gland virus) infection of human cells in tissue culture. Exp. molec. Path.4, 1–10 (1965).
Nii, S., C. Morgan, andH. M. Rose: Electron microscopy of herpes simplex virus. II. Sequence of development. J. Virol.2, 517–536 (1968).
Palay, S. L., andL. J. Karlin: An electron microscopic study of the intestinal villus. II. Pathway of fat absorption. J. biophys. biochem. Cytol.5, 373–384 (1959).
Robertson, J. D.: The molecular structure and contact relationships of cell membranes. Progr. Biophys.10, 343–418 (1959).
Schulze, P., undE. Benndorf: Elektronenmikroskopische Untersuchungen am Aujeszky-Virus in der Gewebekultur. Arch. exp. Vet.-Med.17, 623–631 (1963).
Schwartz, J., andB. Roizman: Similarities and differences in the development of laboratory strains and freshly isolated strains of herpes simplex virus in Hep-2 cells: Electron microscopy. J. Virol.4, 879–889 (1969).
Todorov, D. M., P. C. Wilkinson, andR. G. White: Affinity of immunoglobulin for heterologous tissue mast cells. A study with the fluorescent antibody methods. Immunology15, 51–64 (1968).
Toneva, V.: Purification et concentration du virus de la maladie d'Aujeszky par ultracentrifugation et chromatographie sur colonnes. Microscopie electronique du meme virus. Clin. vet. (Milano)88, 289–315 (1965).
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McCracken, R.M., Clarke, J.K. A thin-section study of the morphogenesis of Aujeszky's disease virus in synchronously infected cell cultures. Archiv f Virusforschung 34, 189–201 (1971). https://doi.org/10.1007/BF01242992
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DOI: https://doi.org/10.1007/BF01242992