Summary
Viruses in blood specimens from patients with hemorrhagic nephroso-nephritis were isolated in monolayer cultures of human embryo kidney cells and passaged in these cultures and in primary and continuous swine kidney cell cultures. The presence of viruses was demonstrated by interference with the growth of heterologous cytopathogenic viruses (poliovirus and Sindbis virus) and by direct and indirect immunofluorescence using human convalescent sera and sera from rabbits hyperimmunized with infected cell cultures as sources of antibody.
The specificity of viruses isolated was confirmed by the regular appearance in convalescent sera from patients with HNN of antibodies which inhibited the multiplication of viruses in cell culture and blocked staining by the immunofluorescence method. The viruses isolated were also neutralized and FA staining bloked by homologous immune rabbit sera.
Virus was present in greatest quantity on the 5th day after inoculation of cell cultures. When virus prepared in tissue culture was inoculated into various types of laboratory animals, including African green monkeys, no illness developed except occasionally among newborn white mice.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Smorodintsev, A. A., V. G.Chudakov, and A. V.Churilov: Haemorrhagic Nephroso-Nephritis. Pergamon Press, 1–124 (1959).
Gavrilyuk, B. K., F. S. Noskov, andA. A. Smorodintsev: Demonstration of the Haemorrhagic Nephroso-Nephritis (HNN) Agent in Cell Cultures by Fluorescent Antibody Combined with Albumin-Rhodamine Contrastive Staining. Acta virol.12, 381 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gavrilyuk, B.K., Smorodintsev, A.A. Isolation of hemorrhagic nephroso-nephritis virus in cell cultures. Archiv f Virusforschung 34, 171–178 (1971). https://doi.org/10.1007/BF01242990
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01242990