Summary
Tritium-labeled reovirus type 2 was adsorbed rapidly and irreversibly to HeLa cells at 37°C. However, if adsorption was carried out at 4°C and the virus-cell complex subsequently incubated at 37° C, more than 25% of the labeled particles was rapidly eluted in 5 minutes. The adsorbed virus remaining at 37°C was stable and no further elution occurred. The eluted virions were found to be biologically and physically unaltered and the elution phenomenon was determined to be dependent on the multiplicity of virus used.
In following the fate of the non-eluted adsorbed virion, labeled viral UNA analyzed in sucrose gradients was found to retain its structural integrity throughout the eclipse period. Loss of radioactivity of the viral genome did not occur until about 12 hours after infection, which was several hours after the first appearance of newly-formed progeny.
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This investigation was supported by Grant AI04685 from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health.
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Loh, P.C., Soergel, M. & Oie, H. Growth characteristics of reovirus type 2: The intracellular fate of viral RNA. Archiv f Virusforschung 22, 398–408 (1967). https://doi.org/10.1007/BF01242960
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DOI: https://doi.org/10.1007/BF01242960