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Electrochemical studies of 9,10-anthraquinone interacting with hemoglobin and determination of hemoglobin

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Abstract

Electrochemical investigation of the interaction of 9,10-anthraquinone (AQ) with hemoglobin (HB) on a mercury electrode is reported for the first time. On addition of hemoglobin to an anthraquinone solution, both the reduction and oxidation currents decrease, with increasing peak separation. In the presence of hemoglobin, no new peaks appear, but the electrochemical parameters (standard rate constantk s and diffusion coefficientD) change significantly. Reaction of anthraquinone with hemoglobin forms an electrochemically active complex HB-AQ. The equilibrium constant for this complex is calculated to be 3.27 × 105 l/mol. A satisfactory result has been obtained for the determination of hemoglobin in clinical blood samples.

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Authors and Affiliations

  1. Department of Chemistry, Peking University, 100871, Beijing, P. R. China

    Zhiwei Zhu & Nan-Qiang Li

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  1. Zhiwei Zhu
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  2. Nan-Qiang Li
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Zhu, Z., Li, NQ. Electrochemical studies of 9,10-anthraquinone interacting with hemoglobin and determination of hemoglobin. Mikrochim Acta 130, 301–308 (1999). https://doi.org/10.1007/BF01242920

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  • DOI: https://doi.org/10.1007/BF01242920

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