A new optical sensor for urea determination is presented. It is based on the enzymatic reaction with urease, which is first photoimmobilized with polyacrylamide onto a chemically polymerized polypyrrole (PPy) film. The main advantage of this sensor is that no indicator dye or pH indicator is needed, because PPy itself acts as the support and the indicator. These PPy films show an absorbance spectrum in the near IR range which is pH dependent. The variation of absorbance is thus directly related to the change of pH caused during the enzymatic reaction, which is also dependent on the urea concentration. The linear range of the sensor is from 0.06 to 1 M of urea, which is the common level of urea concentration found in blood and urine samples.
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M. H. Gil, A. P. Piedade, S. Alegret, J. Alonso, E. Martinez, A. Orellana.Biosensors Bioelectron. 1992,7, 645.
N. Das, P. Prabhakar, M. Kayastha, R. C. Srivastava,Biotech. Bioeng. 1997,54, 329.
D. Z. Shen, Q. Kang, X. Y. Wang,Mikrochim. Acta 1997,126, 267.
S. B. Adeloju, S. J. Shaw, G. G. Wallace,Anal. Chim. Acta 1993,281, 611.
S. Luo, D. R. Walt,Anal. Chem. 1989,61, 1069.
C. Stamm, K. Seiler, W. Simon,Anal. Chim. Acta 1993,282, 229.
S. de Marcos, O. S. Wolfbeis,Sensors Mat. 1997,9, 253.
S. de Marcos, O. S. Wolfbeis,Anal. Chim. Acta 1996,334, 149.
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de Marcos, S., Hortigüela, R., Gatbán, J. et al. Characterization of a urea optical sensor based on polypyrrole. Mikrochim Acta 130, 267–272 (1999). https://doi.org/10.1007/BF01242915
- optical sensor