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Characterization of a urea optical sensor based on polypyrrole

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Abstract

A new optical sensor for urea determination is presented. It is based on the enzymatic reaction with urease, which is first photoimmobilized with polyacrylamide onto a chemically polymerized polypyrrole (PPy) film. The main advantage of this sensor is that no indicator dye or pH indicator is needed, because PPy itself acts as the support and the indicator. These PPy films show an absorbance spectrum in the near IR range which is pH dependent. The variation of absorbance is thus directly related to the change of pH caused during the enzymatic reaction, which is also dependent on the urea concentration. The linear range of the sensor is from 0.06 to 1 M of urea, which is the common level of urea concentration found in blood and urine samples.

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Authors and Affiliations

  1. GEAS (Analytical Spectrometry and Sensors Group), Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, E-50009, Zaragoza, Spain

    Susana de Marcos, Rubén Hortigüela, Javier Gatbán & Juan R. Castillo

  2. Instiute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany

    Otto S. Wolfbeis

Authors
  1. Susana de Marcos
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  2. Rubén Hortigüela
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  3. Javier Gatbán
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  4. Juan R. Castillo
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  5. Otto S. Wolfbeis
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de Marcos, S., Hortigüela, R., Gatbán, J. et al. Characterization of a urea optical sensor based on polypyrrole. Mikrochim Acta 130, 267–272 (1999). https://doi.org/10.1007/BF01242915

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  • DOI: https://doi.org/10.1007/BF01242915

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