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Gesteigerte Immunogenität von Plasmamembranen vaccinia virusinfizierter BHK-Zellen

Increased immunogenicity of plasma membranes of BHK-cells infected by vaccinia virus

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Zusammenfassung

Vacciniavirusinduzierte Zelloberflächenveränderungen können durch den zytoziden Effekt virusspezifischer Antikörper auf infizierte Zellen sowie durch die Agglutination virusinfizierter Zellen durch Concanavalin A demonstriert werden. Diese Veränderungen treten zeitlich korreliert mit einer gesteigerten Immunogenität der Wirtszelle 2–3 Stunden nach Infektionsbeginn bereits vor der Produktion reifer infektiöser Viruspartikeln auf. Eine gesteigerte Antikörperproduktion gegen Wirtszellantigene wird auch nach Immunisierung mit isolierten Plasmamembranen vacciniavirusinfizierter Zellen erreicht, während Zellkernfraktionen diesen Effekt nicht auslösen. Bei den zytotoxischen Antikörpern, die zu den 7 S-Immunglobulinen gehören, handelt es sich — wie durch Absorption mit BHK-Zellmembranen gezeigt werden kann — um spezifisch gegen BHK-Zellen gerichtete Antikörper.

Summary

Cell surface alterations induced by vaccinia virus can be demonstrated either by the cytocidal effect of specific antibodies against vaccinia virus on the infected cells or agglutination of the infected cells by Concanavalin A. This change occurs 2–3 hours after infection, that is before any mature particles of vaccinia virus were produced. It coincides with an increased immunogenicity of the host cell. The production of cytotoxic antibodies against host cell antigens was also increased after active immunization with plasma membranes isolated from vaccinia virus infected BHK-cells, whereas the fraction of cell nuclei did not show up this effect. The cytotoxic antibodies which proved to be 7 S immunoglobulines, are specifically directed against BHK-cells as could be shown by absorption tests with membranes of BHK-cells.

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Author notes

  1. G. Bandlow

    Present address: Hygiene-Institut der Universität Göttingen, Bundesrepublik Deutschland

Authors and Affiliations

  1. Hygiene-Institut der Universität Göttingen, Bundesrepublik Deutschland

    U. Koszinowski & R. Thomssen

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  1. G. Bandlow
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  2. U. Koszinowski
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  3. R. Thomssen
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Gefördert mit Hilfe von Forschungsmitteln des Landes Niedersachsen.

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Bandlow, G., Koszinowski, U. & Thomssen, R. Gesteigerte Immunogenität von Plasmamembranen vaccinia virusinfizierter BHK-Zellen. Archiv f Virusforschung 40, 63–71 (1973). https://doi.org/10.1007/BF01242637

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  • DOI: https://doi.org/10.1007/BF01242637

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