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Voltammetric determination of dipeptidyl aminopeptidases I and IV

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Abstract

Dipeptidyl aminopeptidases I and IV have recently found wide practical application (polypeptide sequencing, clinical diagnostics) so it is necessary to extend present methods and work out new ones for determination of their activity. A new, voltammetric method for determination of peptidase activity is proposed, which allows optimization of the determination conditions according to the activity to be measured. The method has some advantages over the spectrophotometric methods, such as applicability to opaque solutions and higher sensitivity.

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Authors and Affiliations

  1. Instytut Chemii, Wyższa Szkoła Pedagogiczna, ul. Oleska 48, 45-951, Opole, Poland

    Józef Hurek, Małgorzata Bulińska & Teresa Gąsiorowska

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  1. Józef Hurek
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  2. Małgorzata Bulińska
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  3. Teresa Gąsiorowska
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Hurek, J., Bulińska, M. & Gąsiorowska, T. Voltammetric determination of dipeptidyl aminopeptidases I and IV. Mikrochim Acta 108, 311–322 (1992). https://doi.org/10.1007/BF01242442

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  • DOI: https://doi.org/10.1007/BF01242442

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