Summary
Strains of the virus of foot-and-mouth disease obtained from different hosts and tissue cultures were tested in tissue cultures of pig, calf, ox and lamb kidneys for their ability to multiply and produce cytopathogenic effects. It was found that whereas cattle and kidney strains of the virus multiplied well in the cultures with cytopathogenic effect, mouse and egg adapted strains did not multiply or show cytopathogenic effect to the same extent especially in the ox and calf kidneys, and this could be correlated with their behaviour in cattle, pigs and cattle tongue epithelium tissue cultures. With all the strains used it was found possible to produce plaques on pig kidney monolayers, but the size and shape of the plaque varied as well as the relation of plaque titre to the titre in mice. The plaque size and plaque population from different sources were compared, and it was found that the relative number of the different plaque sizes varied with the source of the virus and changed in passage in the different systems. The possible significance of these findings in relation to vaccine preparation and adaptation of the virus is discussed.
Similar content being viewed by others
Bibliography
Brooksby, J. B.: J. Hyg. Camb.48, 184 (1950).
Sellers, R. F.: Nature, London176, 547 (1955).
Bachrach, H. L., W. R. Hess, andJ. J. Callis: Science122, 1269 (1955).
Sellers, R. F. andD. L. Stewart: Arch. ges. Virusforsch.9, 594 (1959).
Skinner, H. H.: Proc. roy. Soc. Med.44, 1041 (1951).
Sellers, R. F.: Proc. roy. Soc. Med.50, 915 (1957).
Wesslén, T. andZ. Dinter: Nord. Vet. Med.8, 795 (1956).
Pay, T. W. F.: Proc. roy. Soc. Med.50, 919 (1957).
Brown, F., R. F. Sellers, andD. L. Stewart: Nature, London182, 535 (1958).
Author information
Authors and Affiliations
Additional information
Part of the work described represents the kidney tissue culture side of experiments on attenuated strains of the virus of foot-and-mouth disease. We are grateful to our colleagues at Pirbright for the supply of virus strains and the results of many cattle, egg and mouse titrations.
We would also like to thank MissP. Tremayne-Smitli, Mr.W. Chapman and Mr.D. Maskell for their excellent assistance in this work.
Rights and permissions
About this article
Cite this article
Sellers, R.F., Burt, L.M., Cumming, A. et al. The behaviour of strains of the virus of foot-and-mouth disease in pig, calf, ox and lamb kidney tissue cultures. Archiv f Virusforschung 9, 637–646 (1960). https://doi.org/10.1007/BF01242149
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01242149