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Factors affecting the formation of plaques by the virus of foot-and-mouth disease on pig kidney monolayer tissue cultures

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Summary

An account is given of some of the factors which may affect the formation of plaques by the virus of foot-and-mouth disease on pig kidney monolayers. The number, size and shape of plaques may be affected by:

  1. 1.

    the previous history and state of the kidney

  2. 2.

    the cultural conditions during growth of the cells

  3. 3.

    the concentration of cells in the monolayer

  4. 4.

    the washing of cultures before and after addition of the virus

  5. 5.

    the period of adsorption

  6. 6.

    the presence and concentration of neutral red in the overlay

  7. 7.

    the conditions under which the overlay is incubated.

The cell suspension method was found more sensitive in virus detection than the monolayer method but used more cells. Any adverse factors tended to depress to a greater extent the plaque count and plaque size of viruses less adapted to pig kidney tissue culture than those well adapted. These findings are discussed from the point of view of the cell and of the virus.

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Author notes

  1. R. F. Sellers

    Present address: Wellcome Research Laboratories, Beckenham, Kent

Authors and Affiliations

  1. Research Institute (Animal Virus Diseases), Pirbright, Surrey

    R. F. Sellers & Doreen L. Stewart

Authors
  1. R. F. Sellers
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  2. Doreen L. Stewart
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Additional information

We are grateful to MissP. Tremayne Smith, Mr.W. Chapman and Mr.M. Maskell for their assistance in the work.

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Sellers, R.F., Stewart, D.L. Factors affecting the formation of plaques by the virus of foot-and-mouth disease on pig kidney monolayer tissue cultures. Archiv f Virusforschung 9, 594–605 (1960). https://doi.org/10.1007/BF01242146

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  • DOI: https://doi.org/10.1007/BF01242146

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