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The agglutination of chicken erythrocytes with special reference to avian histone

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Summary

Attempts were made to fractionate highly purified PR8 influenza virus with the object of finding a chemical basis for the hemagglutinating capacity of this virus. Separate protein and lipid fractions were obtained, but the protein fractions proved to be inactive in agglutinating chicken red cells, and the lipid caused only a mild, pattern-type of agglutination at high concentrations.

An investigation was also made of model agglutinating substances. About a hundred substances were tested for ability to causemajor clumping of chicken red cells at pH 7 in 0.1 M phosphate buffer. Histone preparations obtained from chicken red cells were found to have by far the highest agglutinating capacity of the non-viral substances tested. The histone preparations were characterized with respect to size and shape of constituent particles. elementary and amino acid contents, and sensitivity, relative to PR8 influenza virus, to treatment with ultraviolet light and certain oxidizing agents.

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Bibliography

  1. Hirst, G. K.: The nature of hemagglutination by viruses. The Harvey Lectures, 1948, 1949, Ser. XLIV, p. 84–98, Charles C. Thomas.

  2. Hirst, G. K.: Hemagglutination as applied to the study of virus infection. Viruses 1950, p. 44–51, M. Delbrück, editor, California Institute of Technology.

  3. Burnet, F. M.: The interaction of virus and cell surface. Proc. roy. Soc, Lond., Ser. B. Biol. Sci.138, 47–64 (1951).

    Article  CAS  Google Scholar 

  4. Burnet, F. M.: Mucoproteins in relation to virus action. Physiol. Rev. (Am.)31, 131–150 (1951).

    CAS  Google Scholar 

  5. Hallauer, C.: Die Hämagglutination durch Virusarten im Handbuch der Virus-forschung, 2. Ergänzungsband, p. 141–220.1950.

  6. Sabin, A. B.: Hemagglutination by viruses affecting the human nervous system. Feder. Proc. (Federation of American Societies for Experimental Biology)10, 573–581 (1951).

    CAS  Google Scholar 

  7. Yager, R. H.: Hemagglutination by GDVII and the COL SK, COL MM, mengo encephalomyelitis and encephalomyocarditis viruses. Feder. Proc.10, 579–581 (1951).

    CAS  Google Scholar 

  8. Knight, C. A.: The nucleic acid and carbohydrate of influenza virus. J. exper. Med. (Am.)85, 99–116 (1947).

    Article  CAS  Google Scholar 

  9. Knight, C. A.: Ammo acid composition of highly purified viral particles of influenza A and B. J. exper. Med. (Am.)86, 125–129 (1947).

    Article  CAS  Google Scholar 

  10. Landsteiner, K.: The specificity of serological reactions. Harvard University Press, p. 4–6, 1945.

  11. Hirst, G. K. andE. G. Pickels: A method for the titration of influenza hemagglutinins and influenza antibodies with the aid of a photoelectric densitometer. J. Immunol. (Am.)45, 273–283 (1942).

    Google Scholar 

  12. Miller, G. L. andW. M. Stanley: Quantitative aspects of the red blood cell agglutination test for influenza virus. J. exper. Med. (Am.)79, 185–195 (1944).

    Article  CAS  Google Scholar 

  13. Chargaff, E. andM. Ziff: The compounds between phosphatides and basic proteins. J. biol. Chem. (Am.)131, 25–34 (1939).

    CAS  Google Scholar 

  14. Taylor, A. R.: Chemical analysis of the influenza viruses A (PR8 strain) and B (Lee strain) and the swine influenza virus. J. biol. Chem. (Am.)153, 675–686 (1944).

    CAS  Google Scholar 

  15. Parpart, A. K. andA. J. Dziemian: The chemical composition of the red cell membrane. Cold Spring Harbor Symposia on Quantitative Biology8, 17–24 (1940).

    Article  CAS  Google Scholar 

  16. Ackermann, D.: Zur Chemie der Vogelblutkerne. Hoppe-Seylers Z.43, 299–304 (1904).

    Article  CAS  Google Scholar 

  17. Inone, S.: Ricin. J. Soc. chem. Ind. (Japan) 40, Suppl. bind., p. 122–123, 1937; Chem. Abst.31, 6684 (1937).

    Google Scholar 

  18. Lajmanovich, S. andN. Mittelman: Agglutinating properties of some histones and protamines. Rev. Inst. bacter. “Carlos G. Malbran” (Buenos Aires)12, 320–331 (1944).

    CAS  Google Scholar 

  19. Knight, C. A.: The nature of some of the chemical differences among strains of tobacco mosaic virus. J. biol. Chem. (Am.)171, 297–308 (1947).

    CAS  Google Scholar 

  20. Daly, M. M., A. E. Mirsky, andH. Ris: The amino acid composition and some properties of histones. J. gen. Physiol. (Am.)34, 439–450 (1951).

    Article  CAS  Google Scholar 

  21. Hamer, D.: Amino acid composition of thymus histone. Nature (Brit.)167, 40 (1951).

    Article  CAS  Google Scholar 

  22. Brunish, R., D. Fairley, andJ. M. Luck: Composition of histone prepared from rat liver desoxypentosenucleoproten. Nature (Brit.)168, 82 (1951).

    Article  CAS  Google Scholar 

  23. Knight, C. A.: A sedimentable component of allantoic fluid and its relationship to influenza viruses. J. exper. Med. (Am.)80, 83–100 (1944).

    Article  CAS  Google Scholar 

  24. Burnet, F. M. andW. C. Boake: The relationship between the virus of infectious ectromelia of mice and vaccinia virus. J. Immunol. (Am.)53, 1–13 (1946).

    CAS  Google Scholar 

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  1. Virus Laboratory, University of California, Berkeley, California

    C. A. Knight

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  1. C. A. Knight
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Knight, C.A. The agglutination of chicken erythrocytes with special reference to avian histone. Archiv f Virusforschung 4, 649–658 (1952). https://doi.org/10.1007/BF01242029

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