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Purification studies of rubella virus

Distribution of infectivity in equilibrium centrifugation of potassium citrate density gradients and after chromatography on anion exchanger

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Summary

Equilibrium centrifugation of rubella virus in potassium citrate density gradients revealed a heterogeneous population of virus particles, with 90% of the infectivity being recovered at a density of 1.20g/cm3 and the remaining 10% at 1.10 g/cm3. Pretreatment of the virus material with 1% Tween 80 gave a homogeneous virus peak at 1.20 g/cm3, presumably by splitting aggregates of virus and cell debris.

Chromatography through DEAE Sephadex A25 revealed two distinctly different virus fractions, with 10% appearing in the void volume and 90% being eluted with 0.3–0.5M MgSO4. These fractions were shown not to be influenced by pretreatment with Tween.

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Author notes

  1. Pernilla Magnusson

    Present address: Department of Virus Research, Karolinska Institutet, Stockholm, Sweden

Authors and Affiliations

  1. Department of Virus Research, Karolinska Institutet, Stockholm, Sweden

    Per Skaaret

Authors
  1. Pernilla Magnusson
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  2. Per Skaaret
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Additional information

This work was supported by grant Nr. 65-77 from the Swedish Foundation for Cancer Research.

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Magnusson, P., Skaaret, P. Purification studies of rubella virus. Archiv f Virusforschung 20, 374–382 (1967). https://doi.org/10.1007/BF01241955

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  • DOI: https://doi.org/10.1007/BF01241955

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