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Complement fixation kinetic studies with dengue viruses

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Summary

A complement fixation kinetics test is described for dengue viruses employing antisera obtained from a variety of sources using the standard NCDC micro CF test with parameters that included various time periods of antigen-antibody-C' interaction and various concentrations of antigen.

Antibody to the homotypic dengue serotype is detected earliest during the course of the kinetics. A progressive increase in homologous titers occurred followed thereafter by the detection of cross-reacting CF antibodies. The initial time of antibody detection was dependent upon the antigen-binding capacity of the serum as well as the antigen concentration employed. Irrespective of the antigen concentration, homologous peak titers were achieved only after overnight incubation; however, they were concurrent with heterologous CF antibodies.

Implementation of this procedure facilitated the differentiation of dengue viruses as to type and sometimes as to strain; thus permitting identification which was not possible by the conventional serological procedure.

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Author notes

  1. B. H. Sweet

    Present address: Public Health Science Division, Gulf South Research Institute, New Orleans, Louisiana, USA

Authors and Affiliations

  1. Public Health Science Division, Gulf South Research Institute, New Orleans, Louisiana, USA

    John N. Hatgi

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  1. John N. Hatgi
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  2. B. H. Sweet
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Additional information

This work was supported by U.S.P.H.S. Grant No. 4 R 22-A 1108208, and Contract No. PH-43-68-674.

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Hatgi, J.N., Sweet, B.H. Complement fixation kinetic studies with dengue viruses. Archiv f Virusforschung 35, 323–338 (1971). https://doi.org/10.1007/BF01241913

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  • DOI: https://doi.org/10.1007/BF01241913

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