Summary
Polyoma virus multiplies inin vitro cultures of mouse embryo fibroblasts, and the replicating cycle is accompanied by an increase in the incorporation of labelled precursors into the nucleic acids of the host cells particularly of DNA. From polyoma-infected mouse cells a virus-specific DNA can be isolated which, after purification by chromatography, retains its infectivity forin vitro cultures and oncogenic properties for suckling rodents. Fractionation on a column of methylated albumin allows separation of such virus type DNA from cellular DNA.
Rat embryo cells infected with the oncogenic virus do not replicate the particles or form viral antigen, but undergo a malignant transformation. In this case no evident quantitative change or characteristic qualitative alteration in nucleic acids metabolism of the host cells was observed. Though these data do not offer absolute proof that the alternative pathways induced by polyoma virus, i. e., multiplication of viral particles and malignant transformation, involve two different metabolic pictures in the host cell, they suggest this possibility.
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Cocito, C., Vandeputte, M. & De Somer, P. Alterations in nucleic acid metabolism induced by infection with an oncogenic virus. Archiv f Virusforschung 15, 402–412 (1965). https://doi.org/10.1007/BF01241767
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DOI: https://doi.org/10.1007/BF01241767