Summary
The poliovirus neutralizing antibodies of human colostrum and milk have been identified as belonging to the beta2A class of immunoglobulins. This has been done by isolating this fraction (which is the major one) of the immunoglobulins of colostrum and milk and by showing that the antibody activity was localized in it. The isolation of the beta2A globulins was performed by a combination of preparative electrophoresis or gel-filtration and DEAE column chromatography; the purity of the resulting fraction was checked by immunodiffusion against suitable antisera.
In contrast with the findings in colostrum and milk the bulk of the poliovirus neutralizing antibodies in the serum of the same women, appeared to be localized in the gamma2 globulins, with only minor amounts in the beta2A; it is therefore considered likely that the beta2A globulins (and antibodies) are actively secreted by the mammary gland. In consideration of the fact that beta2A antibodies have a strong affinity for binding to the cells, it is possible that the beta2A poliovirus neutralizing antibodies of human colostrum and milk have some importance in protecting the cells of the alimentary tract of the newborn from the virus infection.
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Giovanardi, A., Pernis, B., Albano, A. et al. Molecular properties of poliovirus neutralizing antibodies in human colostrum and milk. Archiv f Virusforschung 15, 356–365 (1965). https://doi.org/10.1007/BF01241763
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DOI: https://doi.org/10.1007/BF01241763