Skip to main content
SpringerLink
Log in

Quantitative studies on the quality, effects of aggregation and thermal inactivation of vesicular stomatitis virus

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

Quality measurements (number of virions per plaque forming units) were made on vesicular stomatitis virus indicating a virion: p.f.u. ratio of approximately 1. This highly infectious virus was produced by careful control of the inoculum size to avoid the production of the small inhibitory particles associated with VSV. The effect of virus aggregation on titer was investigated, as well as the effect of the dispersal techniques on viral infectivity. Thermal inactivation studies at various temperatures ranging from −57°C to 56°C showed the virus to be relatively stable when stored as a cell lysate or diluted 1∶:10 in nutrient media.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bradish, C. J., J. B. Brooksby, andJ. F. Dillon, Jr.: Biophysical studies of the system of vesicular stomatitis. J. gen. Microbiol.14, 290–314 (1956).

    PubMed  Google Scholar 

  2. Cantell, K., S. Skurska, K. Paucker, andW. Henle: Quantitative studies on viral interference in suspended L-cells. II. Factors affecting interference by UV-irradiated Newcastle disease virus. Virology17, 312–323 (1962).

    PubMed  Google Scholar 

  3. Chow, T. L., F. H. Chow, andR. P. Hanson: Morphology of vesicular stomatitis virus. J. Bact.68, 724–726 (1954).

    PubMed  Google Scholar 

  4. Cooper, P. D., andA. J. D. Bellett: A transmissible interfering component of vesicular stomatitis virus preparations. J. gen. Microbiol.21, 485–497 (1959).

    PubMed  Google Scholar 

  5. Crick, J., B. Cartwright, andF. Brown: Interfering component of vesicular stomatitis virus. Nature (Lond.)211, 1204–1205 (1966).

    Google Scholar 

  6. Galasso, G. J.: Enumeration of VSV particles and a demonstration of their growth kinetics by electron microscopy. Proc. Soc. exp. Biol. (N. Y.)124, 43–48 (1967).

    Google Scholar 

  7. Galasso, G. J., andD. G. Sharp: Virus particle aggregation and the plaque forming unit. J. Immunol.88, 339–347 (1962).

    PubMed  Google Scholar 

  8. Hackett, Adeline J.: A possible morphologic basis for the autointerference phenomenon in vesicular stomatitis virus. Virology24, 51–59 (1964).

    PubMed  Google Scholar 

  9. Hiatt, C. W.: Kinetics of the inactivation of viruses. Bact. Rev.28, 150–163 (1964).

    PubMed  Google Scholar 

  10. Howatson, A. F., andG. F. Whitmore: The development and structure of vesicular stomatitis virus. Virology16, 466–478 (1962).

    PubMed  Google Scholar 

  11. Huang, Alice S., andR. R. Wagner: Defective T. particles of vesicular stomatitis virus II. Biologic role in homologous interference. Virology31, 173–181 (1966).

    Google Scholar 

  12. Isaacs, A.: Particle counts and infectivity titrations for animal viruses. Advanc. Virus Res.4, 111–158 (1957).

    Google Scholar 

  13. McCombs, R. M., M. Benyesh-Melnick, andJ. P. Brunschwig: Biophysical studies of vesicular stomatitis virus. J. Bact.91, 803–812 (1966).

    Google Scholar 

  14. McSharry, J., andG. J. Galasso: Kinetics of vesicular stomatitis virus replication in Earle's L-cells. Bact. Proc.67, 166 (1967).

    Google Scholar 

  15. Philipson, L.: The early interaction of animal viruses and cells. Progr. med. Virol.5, 43–78 (1963).

    Google Scholar 

  16. Sharp, D. G.: Sedimentation counting of particles via electron microscopy. Proc. 4th Int. Congr. Electron Microscopy, Berlin, p. 542–548 (1958).

  17. Sharp, D. G.: Quantitative use of the electron microscope in virus research. Methods and recent results of particle counting. Lab. Invest.14, 831–863 (1965).

    PubMed  Google Scholar 

  18. Williams, R. C., andR. C. Backus: Macromolecular weights determined by direct counting. I. The weight of the bushy stunt virus particle. J. Amer. chem. Soc.71, 4052–4057 (1949).

    Google Scholar 

Download references

Author information

Author notes

  1. G. J. Galasso

    Present address: Department of Microbiology School of Medicine, University of Virginia, Charlottesville, Virg., USA

Authors and Affiliations

    Authors
    1. G. J. Galasso
      View author publications

      You can also search for this author in PubMed Google Scholar

    Rights and permissions

    Reprints and permissions

    About this article

    Cite this article

    Galasso, G.J. Quantitative studies on the quality, effects of aggregation and thermal inactivation of vesicular stomatitis virus. Archiv f Virusforschung 21, 437–446 (1967). https://doi.org/10.1007/BF01241742

    Download citation

    • Received:

    • Issue Date:

    • DOI: https://doi.org/10.1007/BF01241742

    Keywords

    Search

    Navigation