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Segregation of mouse virulent and avirulent ECHO virus type 9 strains and the correlation of mouse-virulence with the “temperature” (rct/40) marker

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Summary

A quantitative study of mouse virulence of ECHO virus type 9 strains, isolated from cases of aseptic meningitis was carried out using the plaque technique. Plaques obtained directly from ECHO virus type 9 isolates exhibited marked variation in mouse virulence; the majority of plaques was of high or low virulence, and only 3 out of 80 plaques tested were completely mouse avirulent. Subplating of these mouse avirulent plaques yielded strains which were predominantly mouse avirulent (45 out of 47 plaques). However, the passage in undiluted form of the avirulent plaque progeny in MKCC and especially in infant mice was selective for mouse virulence.

The rct/40 marker was employed for the study of mouse virulence of the ECHO virus type 9 strains. An excellent correlation was found between the rct/40 marker and mouse virulence; rct/40+ strains were mouse virulent whereas rct/40− strains were avirulent. The rct/40 marker is suggested for use in the determination of mouse virulence of ECHO virus type 9 isolates.

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References

  1. Johnson, T.: A new clinical entity. Lancet272, 590 (1957).

    PubMed  Google Scholar 

  2. Nihoul, E., L. Quersin-Thiry, andA. Weynants: ECHO virus type 9 as the agent responsible for an important outbreak of aseptic meningitis in Belgium. Amer. J. Hyg.66, 102–118 (1957).

    PubMed  Google Scholar 

  3. McLean, D. M., andJ. L. Melnick: Association of mouse pathogenic strain of ECHO virus type 9 with aseptic meningitis. Proc. Soc. exp. Biol. (N. Y.)94, 656–660 (1957).

    Google Scholar 

  4. Quersin-Thiry, L., E. Nihoul, andF. Dekking: ECHO virus type 9 (new member of Coxsackie group type A) as a cause of epidemic meningitis. Science125, 744–745 (1957).

    PubMed  Google Scholar 

  5. Boissard, G. P. B., A. D. Macrae, J. L. Stokes, andF. O. MacCallum: Isolation of viruses related to ECHO virus type 9 from outbreaks of aseptic meningitis. Lancet272, 500–502 (1957).

    Google Scholar 

  6. Ramos-Alvarez, M., andA. B. Sabin: Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. exp. Biol. (N. Y.)87, 655–661 (1954).

    Google Scholar 

  7. Ramos-Alvarez, M., andA. B. Sabin: Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Amer. J. publ. Hlth46, 295–299 (1956).

    Google Scholar 

  8. Rannon, L., N. Goldblum, Z. Leventon, M. Levi, J. Farin, andW. Pushkin: An outbreak of ECHO 9 disease in Israel. Harefuah, J. med. Ass. Israel57, 115–118 (1959).

    Google Scholar 

  9. Goldblum, N., andL. Rannon: Virulence for infant mice of ECHO virus type 9 strains. Bull. Res. Coun., Israel E8, 63–64 (1959).

    Google Scholar 

  10. Ashkenazi, A., andI. Yodfat: An outbreak of an epidemic due to ECHO 9 in a kibbutz. Harefuah, J. med. Ass. Israel67, 289–291 (1964).

    Google Scholar 

  11. Godtfredsen, A., andH. von Magnus: Isolation of ECHO virus type 9 from cerebrospinal fluids. Dan. med. Bull.4, 233–236 (1957).

    PubMed  Google Scholar 

  12. Tyrrell, D. A. J., andB. Snell: Recovery of a virus from cases of an epidemic exanthem associated with meningitis. Lancet271, 1028–1029 (1956).

    PubMed  Google Scholar 

  13. Laforest, R. A., G. A. McNaughton, A. J. Beale, M. Clarke, N. Davis, I. Sultanian, andA. J. Rhodes: Outbreak of aseptic meningitis (meningo-encephalitis) with rubelliform rash: Toronto, 1956. Canad. med. Ass. J.77, 1–4 (1957).

    PubMed  Google Scholar 

  14. Eggers, H. J., andA. B. Sabin: Factors determining pathogenicity of variants of ECHO 9 virus for newborn mice. J. exp. Med.110, 951–967, (1959).

    PubMed  Google Scholar 

  15. Sabin, A. B., E. R. Krumbiegel, andR. Wigand: ECHO type 9 virus disease. Virologically controlled clinical and epidemiologic observations during 1957 epidemic in Milwaukee with notes on concurrent similar diseases associated with Coxsackie and other ECHO viruses. Amer. J. Dis. Child.96, 197–219 (1958).

    Google Scholar 

  16. Wigand, R., andA. B. Sabin: Properties of epidemic strains of ECHO type 9 virus and observations on the nature of human infection. Arch. ges. Virusforsch.11, 683–707 (1962).

    PubMed  Google Scholar 

  17. Lwoff, A.: Factors influencing the evolution of viral diseases at the cellular level and in the organism. Bact. Rev.23, 109–124 (1959).

    PubMed  Google Scholar 

  18. Sabin, A. B.: Reproductive capacity of polioviruses of diverse origins at various temperatures. “Perspectives in Virology” Vol. II. Burgess, Minneapolis, 90–108 (1961).

    Google Scholar 

  19. Dulbecco, R., andMarguerite Vogt: Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med.99, 167–182 (1954).

    PubMed  Google Scholar 

  20. Youngner, J. S.: Virus yield per cell in monkey kidney cultures inoculated with different strains of poliomyelitis virus. J. Immunol.76, 50–53 (1956).

    PubMed  Google Scholar 

  21. Reed, L. J., andH. A. Muench: A simple method of estimating fifty percent end point. Amer. J. Hyg.27, 493–497 (1938).

    Google Scholar 

  22. Miriam Margalith, Eva Margalith, andN. Goldblum: Unpublished results.

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Author notes

  1. N. Goldblum

    Present address: Department of Virology, Hebrew University-Hadassah Medical School, Jerusalem

Authors and Affiliations

  1. Department of Virology, Hebrew University-Hadassah Medical School, Jerusalem

    Miriam Margalith, Eva Margalith, Lotte Rannon, Tamar Goldblum & Sophie Leventon-Kriss

  2. Virus Laboratory, Ministry of Health, Tel-Aviv-Yafo, Israel

    Miriam Margalith, Eva Margalith, Lotte Rannon, Tamar Goldblum, Sophie Leventon-Kriss & N. Goldblum

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  1. Miriam Margalith
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  2. Eva Margalith
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  3. Lotte Rannon
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  4. Tamar Goldblum
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  5. Sophie Leventon-Kriss
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  6. N. Goldblum
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Margalith, M., Margalith, E., Rannon, L. et al. Segregation of mouse virulent and avirulent ECHO virus type 9 strains and the correlation of mouse-virulence with the “temperature” (rct/40) marker. Archiv f Virusforschung 21, 403–412 (1967). https://doi.org/10.1007/BF01241739

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  • DOI: https://doi.org/10.1007/BF01241739

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