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Behaviour of rubella virus in human diploid cell strains II. Studies of infected cells

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Summary

Rubella virus infection of human diploid cells did not result in an ordinary cytopathic effect, but several kinds of human diploid cell strains (HDCS) responded to infection by ceasing to multiply. Inhibition occurred quickly (most lung strains), or after many weeks (skin and pharyngeal mucosa strains). HDCS could be classified according to their response. The response of lung cell strains was strictly related to thein vitro age of the strains at the time of infection. Older strains stopped multiplying several times more quickly than younger ones. The relationship was not found for HDCS of other organ and tissue origins. Pericardial cell strains possessed large amounts of acid phosphatase-containing granules in a small proportion of the cells. During chronic rubella infection, histochemical activity of the enzyme decreased. The chromosomes of chronically infected skin, lung, pharyngeal mucosa and pericardial strains, infected up to 17 cell generations earlier, showed a mild but definite increase in achromatic gaps and breaks. No evidence of cell transformation or induction of neoplastic changes was found in HDCS which had been actively dividing for up to 27 weeks while infected with rubella.

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Author notes

  1. J. M. Hoskins

    Present address: Swiss Serum and Vaccine Institute, Bern, Switzerland

Authors and Affiliations

  1. Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania, USA

    J. M. Hoskins & S. A. Plotkin

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  1. J. M. Hoskins
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  2. S. A. Plotkin
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Additional information

This investigation was supported in part by USPHS Research Grant AI-01799-09 from the National Institute of Allergy and Infectious Diseases and the Joseph P. Kennedy, Jr., Foundation.

Holder of USPHS International Postdoctoral Research Fellowship.

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Hoskins, J.M., Plotkin, S.A. Behaviour of rubella virus in human diploid cell strains II. Studies of infected cells. Archiv f Virusforschung 21, 296–308 (1967). https://doi.org/10.1007/BF01241731

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  • DOI: https://doi.org/10.1007/BF01241731

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