Summary
The nonpathogenic red plaque mutant of Newcastle disease virus (NDV/R) resembled the wild type NDV/W in its antigenic reactivity, hemagglutinin content, UV susceptibility and adsorption-elution pattern with red blood cells. Both viruses showed the same single cycle growth curves. However, in contrast to NDV/W, NDV/R did not induce a high depression of [14C] arginine incorporation into cell proteins, during the early stages of infection.
Low doses of p-fluorophenylalanine (FPA) markedly decreased the cytopathogenic effect of NDV/W, without affecting its multiplication. Similarly, under agar, the plaques caused by NDV/W were phenotypically red, at a certain distance from a paper disk soaked with FPA.
The results suggest that the cytopathogenic effect of NDV/W is related to the synthesis of a protein very susceptible to FPA and not necessary for the maturation of the virus. This protein might play a part in the depression of cellular synthesis.
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Aided by a grant from the “Fonds de la Recherche Scientifique Mé.dicale”.
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Vamos, E. On the “red” character of a mutant of Newcastle Disease Virus (NDV). Archiv f Virusforschung 18, 96–104 (1966). https://doi.org/10.1007/BF01241705
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DOI: https://doi.org/10.1007/BF01241705