Summary
The effects of the receptor destroying enzyme (RDE), potassium periodate and trypsin on the non-specific inhibitors and specific antibodies were studied, employing the haemagglutination inhibition and neutralization tests. It was found that whereas RDE and potassium periodate had no deleterious action on specific antibodies even after 64 hours of treatment, trypsin caused a readily demonstrable decrease in the specific antibody titre already during the first 30 minutes of treatment.
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References
Belyavin, G.: The direct flocculation of influenza virus. Lancet1, 698 (1955).
Belyavin, G.: The influenza virus flocculation reaction as a method of antigenic typing. J. Hyg.55, 281 (1957).
Burnet, F. M., andJ. D. Stone: The receptor-destroying enzyme of Vibrio cholerae. Austr. J. Exp. Biol. Med. Sci.25, 227 (1947).
Chu, C. M., C. H. Andrewes, andA. W. Gledhill: Influenza in 1948–49. Bull. World Health Organ3, 187 (1950).
Chu, C. M.: The action of normal mouse serum on influenza virus. J. Gen. Microbiol.5, 739 (1951).
Cohen, A., andG. Belyavin: Haemagglutination inhibition of asian influenza viruses: A new pattern of response. Virology7, 59 (1959).
Dömök, I., E. Farkas, andM. Gál: Virustani tanulmányok az 1950–51. évi influenza járványról. Népegészségügy12, 1 (1951).
Fazekas de St. Groth, S., J. Withell, andK. J. Lafferty: An improved assay method for neutralizing antibodies against influenza viruses. J. Hyg.56, 415 (1958).
Fazekas de St. Groth, S.: Modification of virus receptors by metaperiodate. I. The properties of JO4 treated red-cells. Aust. J. Exp. Biol. Med. Sci.27, 65 (1949a).
Fazekas de St. Groth, S.: Modification of virus receptors by metaperiodat. II. Infection through modified receptors. Aust. J. Exp. Biol. Med. Sci.27, 83 (1949b).
Francis, T., jr.: Dissociation of hemagglutinating and antibody measuring capacities of influenza virus. J. Exp. Med.85, 1 (1947).
Friedewald, W.: Qualitative differences in the antigenic composition of influenza A virus strains. J. Exp. Med.79, 633 (1944).
Fulton, F., andK. R. Dumbell: The serological comparison of strains of influenza virus. J. Gen. Microbiol.3, 97 (1949).
Ginsberg, H. S., andF. L. Horsfall jr.: A labil component of normal serum which combines with various viruses. Neutralization of infectivity and inhibition of hemagglutination by the component. J. Exp. Med.90, 476 (1949).
Gorbunova, A. S. et al.: Vop. Virusol.2, 21 (1956).
Gorev, N. E.: The use of broth culture filtrates of Pseudomonas fluorescens for the destruction of non-specific thermostabile (56° C) influenza virus inhibitors in human and animal sera. Acta Virologica2, 171 (1958).
Harboe, A., R. Reenaas, andM. Oppedal: Studies on Francis inhibitor of influenza virus haemagglutination in rabbit serum fractions obtained by electrophoreses on starch grain. Acta Path. Microbiol. Scand.44, 92 (1958).
Hilleman, M. R., andF. L. Horsfall jr.: Comparison of the antigenic patterns of influenza A virus strains determined by in ovo neutralization and hemagglutination-inhibition. J. Immunol.69, 343 (1952).
Hirst, G. K.: The quantitative determination of influenza virus and antibodies by means of red cells agglutination. J. Exp. Med.75, 49 (1942).
Hirst, G. K.: In vivo titrations of influenza virus and of neutralizing antibodies in chick embryos. J. Immunol.45, 285 (1942a).
Horváth, S.: A new sensitive method of the rolling drum type for influenza virus titration. Acta Microbiol. Hung.1, 481 (1954).
Horváth, S., andV. Balázs: Virusologische Untersuchungen in Verbindung mit der asiatischen Influenzaepidemie in Szeged. Zeitsch. ges. Inn. Med. Grenz.14, 644 (1959).
James, S. M., andP. Fiset: Serum inhibitors of asian strains of influenza virus. Nature184, 1656 (1959).
Jensen, K. E., andT. Francis jr.: Antigen-antibody precipitates in solid medium with influenza virus. J. Immunol.70, 321 (1953).
Lief, F. S., M. Ostapiak, A. Fabiyi, andW. Henle: Antigenic analysis of influenza viruses by complement fixation. III. Rapid identification of new isolates. J. Immunol.81, 478 (1958).
Lim, K. A., A. Smith, J. H. Hale, andJ. Glass: Influenza outbreak in Singapore. Lancet2, 791 (1957).
Takátsy, Gy., J. Fürész, andE. Farkas: Studies on the quantitative relationships of influenza virus antibody union by means of a simple antibody adsorption test. Acta Physiol. Hung.5, 241 (1954).
Takátsy, Gy.: The use of spiral loops in serological and virological micro-methods. Acta Microbiol. Hung.3, 191 (1955).
Tahátsy, Gy., andK. Barb: On the normal serum inhibitor for the avid asian strains of influenza virus. Acta Virologica3, 71 (1959).
Sampaio, A. A. de C., andA. Isaacs: The action of trypsin on normal serum inhibitors of influenza virus agglutination. Brit. J. Exp. Path.34, 152 (1953).
Smith, W., J. C. N. Westwood, andG. Belyavin: Influenza. A study of four virus strains isolated in 1951. Lancet2, 1189 (1951).
Zhdanov, V. M., V. P. Hamburg, andG. J. Svet-Moldavszky: Antigenicity of the inhibitor of influenza virus strain A (Asia) 57. J. Immunol.82, 9 (1959).
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Horváth, S. Comparison of the experimental results obtained in the course of inactivation of non-specific inhibitors in serum by means of the haemagglutination inhibition and virus neutralization tests. Archiv f Virusforschung 11, 295–303 (1961). https://doi.org/10.1007/BF01241693
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DOI: https://doi.org/10.1007/BF01241693