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Monkeypox virus (MPV) II. Uncoating and nucleic acid synthesis

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Summary

The percentage of uncoating of monkeypox virus (MPV) in CV-1 cells measured by sensitivity of viral DNA to deoxyribonuclease was 24, 46, 53, 48, and 54, at respective 2, 3, 4, 5, and 6 hours post infection. Cores began to appear 1/2 to 1 hour post infection and subsided 3–4 hours post infection to yield naked DNA. The uncoating process was not inhibited by cytosine arabinoside. In cells pretreated with actinomycin-D and infected with MPV in the presence of 2 μg/ml actinomycin-D virion and core peaks were observed but no naked DNA appeared. The time required for a single step growth curve was 12 hours. At 2 hours post infection RNA slightly smaller than 16 S was synthesized. At 4 hours post infection 10 S to 20 S RNA was synthesized; 22 S at 6 to 10 hours. Nuclear RNA synthesis was suppressed.

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Author notes

  1. H. Rouhandeh

    Present address: Laboratory of Molecular and Cancer Virology, Department of Microbiology, Southern Illinois University, Carbondale, Illinois, USA

Authors and Affiliations

  1. Laboratory of Molecular and Cancer Virology, Department of Microbiology, Southern Illinois University, Carbondale, Illinois, USA

    T. M. Yau

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  1. T. M. Yau
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  2. H. Rouhandeh
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Additional information

This investigation was supported in part by Public Health Service Research grant No. 5 R01 CA 10724 from the National Cancer Institute and in part by the Cooperative Research in Molecular and Cancer Virology Program at Southern Illinois University. A preliminary account of this work has been presented (Tommy Yau and H. Rouhandeh, Bact. Proe. V 329, 1970).

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Yau, T.M., Rouhandeh, H. Monkeypox virus (MPV) II. Uncoating and nucleic acid synthesis. Archiv f Virusforschung 39, 151–162 (1972). https://doi.org/10.1007/BF01241538

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  • DOI: https://doi.org/10.1007/BF01241538

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