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Density of an arbovirus in relation to its host cells

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Summary

Studies on density of purified Aura virus grown in two different hosts have shown that this does not differ with the host used for its propagation. Virus grown in tissue culture has a mean value of 1.198±0.008 g/ml and virus grown in suckling mouse brain a mean value of 1.193±0.009 g/ml. The statistical analysis does not show significant difference between the mean density of these two virus populations.

From these results it appears that the density of this member of group A of arboviruses does not depend on the host used for its propagation.

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References

  1. Pfefferkorn, E. R., andH. S. Hunter: The source of the RNA and phospholipid of Sindbis vims. Virology20, 446–456 (1963).

    PubMed  Google Scholar 

  2. Pfefferkorn, E. R., andH. S. Hunter: The origin of the protein of Sindbis virus. Virology23, 217–223 (1964).

    PubMed  Google Scholar 

  3. Caliguri, L. A., Klenk, H. D., andP. W. Choppin: The proteins of the parainfluenza virus SV5 I. Separation of virion polypeptides by poliacrylamide gel electrophoresis. Virology39, 460–466 (1969).

    PubMed  Google Scholar 

  4. Klenk, H. D., andP. W. Choppin: Lipids of plasma membranes of monkey and hamster kidney cells of parainfluenza virions grown in these cells. Virology38, 255–268 (1969).

    PubMed  Google Scholar 

  5. Klenk, H. D., andP. W. Choppin: Plasma membrane lipids and parainfluenza virus assembly. Virology40, 939–947 (1970).

    PubMed  Google Scholar 

  6. Stenback, W. A., andD. P. Durand: Host influence on the density of Newcastle disease virus. Virology20, 545–551 (1963).

    PubMed  Google Scholar 

  7. Lascano, E. F., Berria, M. I., andJ. G. Barrera Oro: Morphogenesis of Aura virus. J. Virol.4, 217–282 (1969).

    Google Scholar 

  8. Barrera Oro, J. G., M. Sabattini, andL. Gutman Frugone: Aura, nuevo arbovirus del grupo A para la Republica Argentina. Cienc. e Invest.23, 180–183 (1963).

    Google Scholar 

  9. Martinez Segovia, Z. M. de: Effect of dimethylsulfoxide on the infectivity of Aura virus ribonucleic acid. Arch. ges. Virusforsch.30, 397–400 (1970).

    PubMed  Google Scholar 

  10. Martinez Segovia, Z. M. de, andF. Grazioli: The nucleic acid of Junin virus. Acta virol.13, 264–268 (1969).

    Google Scholar 

  11. Shope, R. E.: The use of a microhemagglutination-inhibition test to follow antibody response, after arthropod-borne virus infection in a comunity of forest animals. An. Microbiol. (Rio de J.) II, parte A, 167–171 (1963).

    Google Scholar 

  12. Lowry, O. H., N. J. Rosebrouch, A. L. Fahr, andR. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).

    PubMed  Google Scholar 

  13. Warren, J., M. L. Weil, S. B. Russ, andH. Jeffries: Purification of certain viruses by use of protamine sulfate. Proc. Soc. exp. Biol. (N. Y.)72, 662–664 (1949).

    Google Scholar 

  14. Thomas, J. B., A. S. Rioker, G. M. Baer, andR. K. Sikes: Purification of fixed rabies virus. Virology25, 271–275 (1965).

    PubMed  Google Scholar 

  15. Black, L. M., D. V. R. Reddy, andM. E. Reichman: Virus inactivation by moderate forces during quasi-equilibrium zonal density gradient centrifugation. Virology31, 713–715 (1967).

    PubMed  Google Scholar 

  16. Akira, I., K. Fukai, andP. Tuchinda: Studies on Chikungunya virus II. Purification of mouse brain virus. Biken J.10, 189–194 (1967).

    PubMed  Google Scholar 

  17. Hoskins, J. M.: Host-controlled variation in animal viruses. Symp. Soc. gen. Microbiol.9, 122–141 (1959).

    Google Scholar 

  18. Schlesinger, R. W.: Incomplete growth cycle of influenza virus in mouse brain. Proc. Soc. exp. Biol. (N. Y.)74, 541–548 (1950).

    Google Scholar 

  19. Henle, G., A. Girardi, andW. Henle: A non-transmisible cytopathogenic effect of influenza virus in tissue culture accompanied by formation of non-infectious hemagglutinins. J. exp. Med.101, 25–41 (1955).

    PubMed  Google Scholar 

  20. Hsiung, G. D.: Studies on variation in Coxsackie A-9 virus. J. Immunol.84, 258–291 (1960).

    PubMed  Google Scholar 

  21. Drake, J. W., andP. A. Lay: Host-controled variation in NDV. Virology17, 56–64 (1962).

    PubMed  Google Scholar 

  22. Simpson, W. R., andR. E. Hauser: Influence of lipids on the viral phenotype. Virology30, 684–697 (1966).

    PubMed  Google Scholar 

  23. Friedman, R. M., andI. Pastan: Nature and function of the structural phospholipids of an arbovirus. J. molec. Biol.40, 107–115 (1969).

    PubMed  Google Scholar 

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Bendersky, S., de Martinez Segovia, Z.M. Density of an arbovirus in relation to its host cells. Archiv f Virusforschung 37, 316–322 (1972). https://doi.org/10.1007/BF01241454

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  • DOI: https://doi.org/10.1007/BF01241454

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