Summary
A procedure for titrating the capacity of poliovirus RNA preparations to interfere with plaque initiation by another poliovirus RNA was developed. Treatment of extractive poliovirus RNA preparations with deoxyribonuclease, trypsin, or chymotrypsin did not affect their interfering capacity; the previously reported decrease in interfering capacity effected by ribonuclease was confirmed and further studied.
Comparisons of profiles of infectivity and interfering capacity in fractions from hydromagnesite columns revealed that, for 3x extractive infective Brunhilde RNA preparations, the major peak of interfering capacity nearly coincided with the major peak of infectivity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rouhandeh, H., andG. R. Dubes: Fed. Proc.23, 507 (1964).
Dubes, G. R., andH. Rouhandeh: Fed. Proc.22, 487 (1963).
Dubes, G. R., andH. Rouhandeh: Arch. ges. Virusforsch.14, 87 (1963).
Faas, F. H., H. Rouhandeh, R. D. Lamb, D. G. Kelly, M. Chapin, T. A. Lucas, andG. R. Dubes: Genetics48, 889 (1963).
Rouhandeh, H., R. Chronister, andM. L. Brinkman: Bact. Proc.5, 144 (1964).
Dubes, G. R., andE. A. Klingler, Jr.: Science133, 99 (1961).
Lamb, R. D., andG. R. Dubes: Analyt. Biochem.7, 152 (1964).
Author information
Authors and Affiliations
Additional information
Aided in part by a grant from the National Foundation and in part by United States Public Health Training Grant No. 2E-137 (C2S1). Part of these results were presented in Chicago, Illinois, on April 16, 1964, at a meeting of the American Association of Immunologists (1).
Rights and permissions
About this article
Cite this article
Rouhandeh, H., Dubes, G.R. A study of the nature of the interfering material in preparations of poliovirus ribonucleic acid. Archiv f Virusforschung 15, 1–6 (1964). https://doi.org/10.1007/BF01241416
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01241416