Skip to main content
SpringerLink
Log in

Morphological and immunological studies on reovirus type 2 hemagglutinin

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

  1. 1.

    Evidence is presented that reovirus hemagglutinin is an integral part of the structurally intact virus regardless of the presence or absence of infectivity. No hemagglutinin apart from that associated with the morphologically intact virus could be demonstrated.

  2. 2.

    Chloroform treatment of reovirus type 2 causes disintegration of the viral capsid with destruction of capsomeres. Furthermore, profound antigenic changes of the viral protein occur after chloroform treatment. Therefore chloroform seems to act on the protein of the virus destroying hemagglutinating activity without affecting infectivity and should therefore not be interchangeably used with ether to determine the presence or absence of “essential lipids” as it is commonly used in classifying viruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rozee, K. R., andW. D. Leers: Chloroform inactivation of reovirus hemagglutinin. Canad. med. Ass. J.96, 597 (1967).

    PubMed  Google Scholar 

  2. Sabin, A. B.: Reoviruses. Science130, 1387 (1959).

    PubMed  Google Scholar 

  3. Gomatos, P. J., I. Tamm, S. Dales, andR. M. Franklin: Reovirus type 3. Physical characteristics aad interactions with L cells. Virology17, 441 (1962).

    PubMed  Google Scholar 

  4. Usmankhodzhayev, A., andL. Y. Zakstelskaya: Stability of reovirus hemagglutinin. Acta virol. (Praha) (Engl.)8, 84 (1964).

    Google Scholar 

  5. Mayr, A., undK. Bögel: Der Chloroformresistenztest zur Isolierung und Charakterisierung von Enteroviren. Zbl. Bakt. I. Abt. Orig.182, 564 (1961).

    Google Scholar 

  6. Feldman, H. A., andS. S. Wang: Sensitivity of various viruses to chloroform. Proc. Soc. exp. Biol. (N.Y.)106, 736 (1961).

    Google Scholar 

  7. Leers, W. D.: “The biological properties of reoviruses with particular reference to antigenic differentiation.” Dissertation. University of Toronto, Toronto, Ontario (Canada) (1967).

    Google Scholar 

  8. Leers, W. D., K. R. Rozee, andA. C. Wardlaw: Immunoelectrophoretic studies of reovirus antigens. Canad. J. Microbiol. (1968) (in press).

  9. Pinteric, L., andJ. Taylor: The lowered drop method for the preparation of specimens of partially purified virus lysates for quantitative electron micrographic analysis. Virology18, 359 (1962).

    Google Scholar 

  10. Crowle, A. T.: Immunodiffusion. New York and London: Academic Press (1961).

    Google Scholar 

  11. Knight, C. A.: Chemistry of viruses. Protoplasmatologia. Band 4. Wien: Springer-Verlag (1963).

    Google Scholar 

  12. Peters, D., G. Müller undR. Geister: Zur Deutung sogenannter „inkompletter“ Formen bei der Negativkontrastierung von Viren. Arch. ges. Virusforsch.12, 437 (1962).

    PubMed  Google Scholar 

  13. Müller, G., C. C. Schneider undD. Peters: Zur Feinstruktur des Reovirus (Typ 3). Arch. ges. Virusforsch.12, 110 (1966).

    Google Scholar 

  14. Zalan, E., andN. A. Labzoffsky: The hemagglutinin of reovirus type 3. Arch. ges. Virusforsch.21, 25 (1967).

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. W. D. Leers (Assistant Professor, Microbiologist)

    Present address: Department of Microbiology, School of Hygiene, Canada

Authors and Affiliations

  1. Department of Microbiology, School of Hygiene, Canada

    K. R. Rozee (Associate Professor)

  2. Department of Bacteriology, Faculty of Medicine, University of Toronto, Canada

    W. D. Leers (Assistant Professor, Microbiologist)

  3. The Wellesley Hospital and Princess Margaret Hospital, Toronto, Ontario

    W. D. Leers (Assistant Professor, Microbiologist)

  4. Department of Microbiology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia

    K. R. Rozee (Associate Professor)

Authors
  1. W. D. Leers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. R. Rozee
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Leers, W.D., Rozee, K.R. Morphological and immunological studies on reovirus type 2 hemagglutinin. Archiv f Virusforschung 24, 155–163 (1968). https://doi.org/10.1007/BF01241288

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01241288

Keywords

Search

Navigation