Summary
Top and bottom components of alfalfa mosaic virus have been separated by combined differential and density gradient centrifugation. Bottom component was obtained in a highly purified state. The advantage of the technique employed is that it allows one to obtain the two viral components in mg quantities. Straight fractionation from zones obtained in centrifuged density-gradient columns yielded relatively pure top-component, but bottom-component still contaminated with top-component and middle-component. Also, much greater effort was required to obtain high yields of either component if differential centrifugation was eliminated from the procedure.
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References
Bancroft, J. B.: Association of infectivity with alfalfa mosaic virus bottom component. Virology14, 296–297 (1961).
Bancroft, J. B., andP. Kaesberg: Size and shape of alfalfa mosaic virus. Nature (Lond.)181, 720–721 (1958).
Bancroft, J. B., andP. Kaesberg: Macromolecular particles associated with alfalfa mosaic virus. Biochim. biophys. Acta (Amst.)39, 519–528 (1960).
Brakke, M. K.: Nonideal sedimentation and the capacity of sucrose gradient columns for virus in density-gradient centrifugation. Arch. Biochem.107, 388–403 (1964).
Brakke, M. K., andJ. M. Daly: Density gradient centrifugation: Non-ideal sedimentation and the interaction of major and minor components. Science148, 387–389 (1965).
Claasen, J. C. v. R., A. B. J. van Leeuwen, G. A. H. Duijts, andL. Bosch:In vitro translation of alfalfa mosaic virus RNA. J. molec. Biol.23, 535–544 (1967).
Diener, T. O., andP. R. Desjardins: Factors affecting the stability of tobacco mosaic virus. I. Ribonuclease-induced protection of virus from alkaline degradation. Virology29, 15–25 (1966).
Frisch-Niggemeyer, W., andR. L. Steere: Chemical composition of partially purified alfalfa mosaic virus. Virology14, 83–87 (1961).
Gibbs, A. J., H. L. Nixon, andR. D. Woods: Properties of purified preparations of Lucerne mosaic virus. Virology19, 441–449 (1963).
Kelley, J. J., andP. Kaesberg: Biophysical and biochemical properties of top component and bottom component of alfalfa mosaic virus. Biochim. biophys. Acta (Amst.)61, 865–871 (1962).
Planterose, D. N., andJ. K. O. Ryan: A 65S particle containing viral protein in cells infected with foot-and-mouth disease virus. Virology26, 372–374 (1965).
Rauws, A. G., E. M. J. Jaspars, andH. Veldstra: The base composition of ribonucleic acids from alfalfa mosaic virus components. Virology23, 283–286 (1964).
Ross, A. F.: Purification and properties of alfalfa-mosaic virus protein. Phytopath.31, 394–410 (1941).
Steere, R. L.: Purification and properties of tobacco ringspot virus. Phytopath.46, 60–69 (1956).
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This research was conducted while the first author was in pursuance of a Postdoctoral Resident Research Associateship with the Agricultural Research Service and on leave from the University of California, Riverside.
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Desjardins, P.R., Steere, R.L. Separation of top and bottom components of alfalfa mosaic virus by combined differential and density gradient centrifugation. Archiv f Virusforschung 26, 127–137 (1969). https://doi.org/10.1007/BF01241182
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DOI: https://doi.org/10.1007/BF01241182