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Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in nebraska

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Summary

The Nebraska calf diarrhea viral (NCDV) agent was successfully adapted and propagated in several cell culture systems. Criteria of propagation were: production of a cytopathic effect, specific fluorescence, interference with cytopathogenicity of another virus, infection of neonatal calves, and identification of the NCDV agent by electron microscopy (EM) or immuno-electron microscopy (I-EM). The EM and I-EM techniques proved to be valuable diagnostic aids for detecting NCDV disease in affected calves.

Morphologically, the NCDV agent resembled the reoviruses. Resistance to lipid solvents and fluorocarbon further related NCDV to this group. The NCDV agent, however, was serologically unrelated to reoviruses so a “reovirus-like” classification for this virus was suggested.

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Author notes

  1. A. L. Fernelius

    Present address: National Animal Disease Laboratory, Veterinary Sciences Research Division, Agricultural Research Service, US Department of Agriculture, Ames, Iowa

  2. L. G. Classick

    Present address: Office of the Extension Veterinarian, Iowa State University, 109 Kildee Hall, 50010, Ames, IA, USA

Authors and Affiliations

  1. National Animal Disease Laboratory, Veterinary Sciences Research Division, Agricultural Research Service, US Department of Agriculture, Ames, Iowa

    A. E. Ritchie, L. G. Classick, J. O. Norman & C. A. Mebus

  2. Department of Veterinary Science, College of Agriculture and Home Economics, University of Nebraska, Lincoln, Nebraska, USA

    A. L. Fernelius, A. E. Ritchie, L. G. Classick, J. O. Norman & C. A. Mebus

Authors
  1. A. L. Fernelius
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  2. A. E. Ritchie
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  3. L. G. Classick
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  4. J. O. Norman
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  5. C. A. Mebus
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Additional information

Published with the approval of the Director as paper No. 3167, Journal Series, Nebraska Agricultural Experiment Station Project No. 14-1. This investigation was done in cooperation with the US Department of Agriculture, Agricultural Research Service, Agreement No. 12-14-100-10, 610 (45).

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Fernelius, A.L., Ritchie, A.E., Classick, L.G. et al. Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in nebraska. Archiv f Virusforschung 37, 114–130 (1972). https://doi.org/10.1007/BF01241157

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  • DOI: https://doi.org/10.1007/BF01241157

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