Skip to main content
SpringerLink
Log in

Infection of chick embryo tracheal organ cultures with influenza A 2 (Hong Kong) virus

I. Cytopathology, histopathology, immunofluorescence, hemadsorption, and titration of the released infectious progeny virus

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

Chick embryo tracheal organ culture was found to be a suitable system for the propagation of Influenza A 2 (Hong Kong) virus. Growth of the virus could be detected by the appearance of hemadsorption and immunofluorescence as early as 12 hours after infection. Cytopathic changes appeared before the cessation of ciliary activity. All of the above mentioned changes were found to be reliable indicators of viral growth and did not occur if influenza A 2 (Hong Kong) virus was mixed with specific antiserum and inoculation was made with this complex.

Comparisons were made with infected primary rhesus monkey kidney cells, the system commonly used for isolation and propagation of influenza A 2 (Hong Kong) virus. The peak virus titre in the infected organ culture fluids was reached in 24 hours after inoculation as compared with 60 hours in primary rhesus monkey kidney cells.

The chick embryo tracheal organ culture system is at least as sensitive as the rhesus monkey kidney cell cultures and is more readily available because of the continuous availability of fertile eggs in every laboratory.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hoorn, B., andD. A. J. Tyrrell: Organ cultures in virology. Progr. med. Virol.11, 408–450 (1969).

    Google Scholar 

  2. Tyrrell, D. A. J., andM. L. Bynoe: Cultivation of a novel type of common cold virus in organ cultures. Brit. med. J.1, 1467–1470 (1965).

    Google Scholar 

  3. Hoorn, B., andD. A. J. Tyrrell: A new virus cultivated only in organ cultures of human ciliated epithelium. Arch. ges. Virusforsch.18, 210–225 (1966).

    Google Scholar 

  4. Almeida, J. D., andD. A. J. Tyrrell: The morphology of three previously uncharacterized human respiratory viruses that grow in organ culture. J. gen. Virol.1, 175–178 (1967).

    Google Scholar 

  5. Hoorn, B.: Respiratory viruses in model experiments. Acta oto-laryng. (Stockh.) Suppl.188, 138–144 (1964).

    Google Scholar 

  6. Hoorn, B., andD. A. J. Tyrrell: On the growth of certain “Newer” respiratory viruses in organ cultures. Brit. J. exp. Path.46, 109–118 (1965).

    Google Scholar 

  7. Bang, F. B., andJ. S. F. Niven: A study of infection in organized tissue cultures. Brit. J. exp. Path.39, 317–322 (1958).

    Google Scholar 

  8. Hoorn, B.: Organ cultures of ciliated epithelium for the study of respiratory viruses. Acta path, microbiol. scand. Suppl.183, 66 (1966).

    Google Scholar 

  9. Blaskovic, P., A. J.Rhodes, F. W.Doane, and N. A.Labzoffsky: Infection of chick embryo tracheal organ cultures with influenza A 2 Hong Kong virus-electron microscopy (Personal observations).

  10. Cherry, J. D., andD. Taylor-Robinson: Large-quantity production of chicken embryo tracheal organ cultures and use in virus and mycoplasma studies. Appl. Microbiol.19, 658–662 (1970).

    Google Scholar 

  11. Good, N. E., G. D. Winget, W. Winter, T. N. Connolly, S. Izawa, andR. M. M. Singh: Hydrogen ion buffers for biological research. Biochemistry,5, 467–477 (1966).

    Google Scholar 

  12. Clark, M. F., andC. C. Shepard: A dialysis technique for preparing fluorescent antibody. Virology20, 642–644 (1963).

    Google Scholar 

  13. Tyrrell, D. A. J., andB. Hoorn: The growth of some myxoviruses in organ cultures. Brit. J. exp. Path.46, 514–518 (1965).

    Google Scholar 

  14. Tyrrell, D. A. J., andC. J. Blamire: Improvements in a method of growing respiratory viruses in organ cultures. Brit. J. exp. Path.48, 217–227 (1967).

    Google Scholar 

  15. McIntosh, K., J. M. Dees, W. B. Becker, A. Z. Kapikian, andR. M. Chanock: Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. nat. Acad. Sci. (Wash.)57, 933–940 (1967).

    Google Scholar 

Download references

Author information

Author notes

  1. N. A. Labzoffsky

    Present address: Virus Laboratory, Laboratory Services, Branch Ontario Department of Health, Terminal A, Box 9000, Toronto 116, Ontario, Canada

Authors and Affiliations

  1. Virus Laboratory, Laboratory Services, Branch Ontario Department of Health, Terminal A, Box 9000, Toronto 116, Ontario, Canada

    P. Blaskovic & A. J. Rhodes

  2. Department of Microbiology, School of Hygiene, University of Toronto, Toronto, Ontario, Canada

    P. Blaskovic, A. J. Rhodes & N. A. Labzoffsky

Authors
  1. P. Blaskovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. J. Rhodes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. A. Labzoffsky
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Submitted in partial fulfilment of the requirements of the degree Master of Science, University of Toronto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Blaskovic, P., Rhodes, A.J. & Labzoffsky, N.A. Infection of chick embryo tracheal organ cultures with influenza A 2 (Hong Kong) virus. Archiv f Virusforschung 37, 104–113 (1972). https://doi.org/10.1007/BF01241156

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01241156

Keywords

Search

Navigation