Summary
CV-1 cells which survived SV 40 infection developed resistance to SV 40 and changes in growth potential and morphology. The changes in growth and resistance to SV 40 were not permanent in the majority of the surviving cells. The number of surviving colonies decreased exponentially over a five week period. During this time, secondary changes in cell morphology took place. Stable cell strains emerged. Although these strains continued to release small quantities of SV 40, an increase in the number of infected cells (containing V antigen) could not be produced by superinfection. An isolated clone of the resistant cells remained free of detectable virus for two months but then spontaneously released virus. Treatment with anti-SV 40 serum reduced the concentration of extracellular virus but not that of intracellular virus. The fraction of cells in which T antigen fluorescence could be found varied from 10–50% but never reached 100%, even after long term cultivation.
The results are consistent with the concept that both the T antigen positive cells and those that continue to release virus arise from T antigen negative, abortively transformed cells in which the viral genome is unstably suppressed.
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Part of these results has been reported at the Third Heidelberg Symposium, Aktuelle Probleme aus dem Gebiet der Cancerologie III, September, 1970.
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Hahn, E.C. The development of CV-1 cells resistant to SV 40. Archiv f Virusforschung 37, 34–44 (1972). https://doi.org/10.1007/BF01241148
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DOI: https://doi.org/10.1007/BF01241148