Summary
Foot-and-mouth disease virus adapted to adult mice was cloned and compared to the same virus after its treatment with a mutagenin vitro. After treatment, a viral clone was isolated that was partially attenuated for adult mice.
The two viral clones were indistinguishable in their inactivation rates with antiserum, urea and iodine, as well as in their susceptibility to urea and guanidine incorporated in the growth medium.
Both clones were equally inhibited in their multiplication on primary bovine kidney cells in the presence of an interferon inducer.
The size of the plaques formed under 1% agar on BHK 21/13 cells was unrelated to the pathogenicity of the virus for adult mice. The plaque size of the attenuated clone was smaller than that produced by the original clone when tested on a cell line of swine kidney origin.
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Maes, R.F. Investigations of the attenuation induced in foot-and-mouth disease virus by a chemical mutagen. Archiv f Virusforschung 37, 19–33 (1972). https://doi.org/10.1007/BF01241147
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DOI: https://doi.org/10.1007/BF01241147