Summary
SIRC cells, which have been shown to support plaque formation of rubella virus, were found to consist of a number of cell clones with different degrees of virus susceptibility. Clones isolated from the original SIRC cell population were classified by their ability to form plaques into 3 groups; namely clones producing 1) clear plaques, 2) pin-hole plaques, and 3) no plaques. It was pointed out that in some clones, these characteristics were relatively unstable during subcultivations. The most favorable concentration of sodium bicarbonate for plaque formation of rubella virus was 0.2–0.4%. The addition of DEAE-dextran prior to viral inoculation enhanced the efficiency of plaquing.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rhim, J. S., andK. Schell: Cytopathic and plaque assay of rubella virus in a line of African green monkey kidney cells (Vero). Proc. Soc. exp. Biol. (N.Y.)125, 602–606 (1967).
Liebhaber, H., J. T. Riobdan, andD. M. Horstman: Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero). Proc. Soc. exp. Biol. (N.Y.)125, 636–643 (1967).
Rhim, J. S., K. Schell, andR. J. Huebner: Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. exp. Biol. (N.Y.)125, 1271–1274 (1967).
Vaheri, A., W. D. Sedwick, andS. A. Plotkin: Growth of rubella virus in BHK 21 cells. I. Production, assay and adaptation of virus. Proc. Soc. exp. Biol. (N.Y.)125, 1086–1092 (1967).
Plotkin, S. A.: Plaquing of rubella virus in RK13 cells. Arch. ges. Virusforsch.16, 422–425 (1965).
Puck, T. T., P. I. Marcus, andS. J. Cieciura: Clonal growth of mammalian cellsin vitro. Growth characteristics of colonies from single HeLa cells with and without a “feeder” layer. J. exp. Med.103, 273–284 (1956).
Parkman, P. D., E. L. Buescher, M. S. Artenstein, J. M. McCown, P. K. Mundon, andA. D. Druzd: Studies of rubella. I. Properties of the virus. J. Immunol.93, 595–607 (1964).
Vogt, M., R. Dulbecco, andH. A. Wenner: Mutants of poliomyelitis viruses with reduced efficiency of plating in acid medium and reduced neuropathogenicity. Virology4, 141–155 (1957).
Khadre, M. A., andJ. Fong: Effect of sodium bicarbonate upon Myxoma virus plaque morphology. Proc. Soc. exp. Biol. (N.Y.)122, 791–795 (1966).
Kondo, A.: Plaque formation of rabies virus in PCE cells. Proceedings of 13th Annual Meeting of Japanese Virologists, 1965 (in Japanese).
Marcus, P. I., andD. H. Carver: Hemadsorption-negative plaque test: New assay for rubella virus revealing a unique interference. Science149, 983–986 (1965).
Vaheri, A., W. D. Sedwick, andS. A. Plotkin: Growth of rubella virus in BHK21 cells. II. Enhancing effect of DEAE-dextran, Semicarbazide and low doses of metabolic inhibitors. Proc. Soc. exp. Biol. (N.Y.)125, 1092–1098 (1967).
Liebhaber, H., andK. K. Takemoto: Alteration of plaque morphology of EMC virus with polycations. Virology14, 502–504 (1961).
Leerhøy, J.: The influence of different media on cell morphology and rubella virus titer in a rabbit cornea cell line (SIRC). Arch. ges. Virusforsch.19, 210–220 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sato, H., Kohno, S. Plaque formation of rubella virus in clones of SIRC cells. Archiv f Virusforschung 28, 399–410 (1969). https://doi.org/10.1007/BF01240953
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01240953