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Interrelated effects of cysteine, thyroxin, and substrate on growth rate of influenza virus in tissue culture

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Summary

In cultures of chorioallantoic membrane in simple balanced salt solution, cysteine at a concentration of 0.5 to 1.0 mg/ml. interferes with the utilization of pyruvate and glutamate for tissue metabolism and growth of influenza virus. Thyroxin stimulates growth of virus and causes partial or complete reversal of the inhibitory effect of cysteine on virus reproduction. When glucose is employed as the substrate, cysteine is much less inhibitory to tissue respiration and virus, and thyroxin is found to be less effective in accelerating virus reproduction. Thyroxin was also found to increase slightly the rate of virus synthesis in tissue cultures partially inhibited with 2,4-dinitrophenol or malonate.

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Authors and Affiliations

  1. Department of Bacteriology and Immunology Harvard Medical School Boston, Mass

    Monroe D. Eaton & Anthony R. Scala

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  1. Monroe D. Eaton
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  2. Anthony R. Scala
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This work was aided by research grant C1657 from the National Cancer Institute, U. S. Public Health Service, and by funds from the Eugene Higgins Trust.

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Eaton, M.D., Scala, A.R. Interrelated effects of cysteine, thyroxin, and substrate on growth rate of influenza virus in tissue culture. Archiv f Virusforschung 7, 274–283 (1957). https://doi.org/10.1007/BF01240880

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  • DOI: https://doi.org/10.1007/BF01240880

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