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Enhancement of MHC-unrestricted cytotoxic activity of human CD56+ CD3− natural killer (NK) cells and CD3+ T cells by rhamnogalacturonan: Target cell specificity and activity against NK-insensitive targets

  • Original Papers
  • Experimental Oncology
  • Published:
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Abstract

Rhamnogalacturonan-mediated enhancement of MHC-unrestricted cytotoxicity was studied with freshly isolated CD56+CD3− natural killer (NK) cells, interleukin-2 (IL-2)-activated CD56+ lymphokine-activated killer (LAK) cells und IL-2/anti-CD3-activated T cells as effector cells using NK-sensitive and NK-insensitive tumor cells as targets. The rhamnogalacturonan fractions IM, IP, and IQ were prepared from commercially available extracts ofViscum album. The dose/response relation of IM, IP, and IQ demonstrated the presence of various concentrations of cytotoxicity-enhancing compounds in all three fractions that were identified as rhamnogalacturonans by degradation studies with poly-α-d-galacturonidase (EC 3.2.1.15) and α-1,6-rhamnosidase (EC 3.2.1.40). Specific cytotoxicity of all three effector cell populations as well as the respective rhamnogalacturonan-mediated cytotoxicity enhancement was readily inhibited in a dose-dependent manner by 60%-deacetylated mannose pentaacetate. Rhamnogalacturonan-mediated enhancement of cytotoxicity of fresh CD56+ NK cells was also observed with four of five NK-insensitive tumor cells as targets, indicating that the effector-cell/tumor-cell bridging activity of rhamnogalacturonans renders NK-insensitive targets susceptible to NK-mediated lysis. Moreover, the rhamnogalacturonan-mediated cytotoxicity enhancement became even more prominent when lymphokine-activated CD56+ LAK and CD3+ T cells were assayed with the NK-insensitive tumor cell targets.

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Abbreviations

E/T:

effector cell/tumor cell ratio

IL:

interleukin

LAK:

lymphokinje-activated killer

LDH:

lactic acid dehydrogenase

MHC:

major histocompatibility complex

NK:

natural killer

PBMC:

peripheral blood mononuclear cells

PNAC:

peripheral non-adherent cells

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Authors and Affiliations

  1. Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Spemannstrasse 37/39, D-72011, Tübingen, Germany

    Hong-Guang Zhu, Thomas M. Zollner, Andreas Klein-Franke & F. Alfred Anderer

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  1. Hong-Guang Zhu
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  2. Thomas M. Zollner
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  3. Andreas Klein-Franke
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  4. F. Alfred Anderer
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Zhu, HG., Zollner, T.M., Klein-Franke, A. et al. Enhancement of MHC-unrestricted cytotoxic activity of human CD56+ CD3− natural killer (NK) cells and CD3+ T cells by rhamnogalacturonan: Target cell specificity and activity against NK-insensitive targets. J Cancer Res Clin Oncol 120, 383–388 (1994). https://doi.org/10.1007/BF01240135

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  • DOI: https://doi.org/10.1007/BF01240135

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